Isolation of cDNA encoding a binding protein specific to 5'-phosphorylated single-stranded DNA with G-rich sequences

Nucleic Acids Res. 1993 Apr 25;21(8):1761-6. doi: 10.1093/nar/21.8.1761.

Abstract

We have isolated the cDNA encoding a binding protein to the sequence motif of the immunoglobulin S mu region by the southwestern method. The binding protein designated S mu bp-2 specifically binds to 5'-phosphorylated single-stranded DNA containing 5'-G and GGGG stretches. The amino acid sequence deduced from the cDNA sequence showed that the S mu bp-2 belongs to the putative helicase superfamily which is involved in replication, recombination and repair. Expression of S mu bp-2 mRNA is ubiquitous and augmented in spleen cells stimulated with lipopolysaccharide and interleukin 4 which also induce class switching. The S mu bp-2 gene is conserved among vertebrates. Possible involvement of S mu bp-2 in class switching is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • DNA, Single-Stranded / chemistry
  • DNA, Single-Stranded / metabolism*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / isolation & purification
  • Guanine / analysis
  • Humans
  • Immunoglobulin Switch Region
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Phosphorylation
  • Recombination, Genetic
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription Factors*

Substances

  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • IGHMBP2 protein, human
  • Ighmbp2 protein, mouse
  • Transcription Factors
  • Guanine

Associated data

  • GENBANK/L10075