Abstract
Transformation of chick embryonic fibroblasts with Rous sarcoma virus strongly suppresses N-cadherin-mediated cell-cell adhesion, without inhibiting its expression. This suppression is correlated with tyrosine phosphorylation of N-cadherin and catenins, the cadherin-associated proteins, which are known to regulate cadherin function. Experiments with non-myristylation and temperature-sensitive mutants of RSV and with herbimycin A, a potent inhibitor of tyrosine kinases, suggest that both the suppression of cell adhesion and tyrosine phosphorylation of catenins are highly transformation-specific.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Avian Sarcoma Viruses / genetics*
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Benzoquinones
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Cadherins / physiology*
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Cell Adhesion / physiology*
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Cell Aggregation
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Cell Transformation, Neoplastic*
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Cells, Cultured
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Chick Embryo
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Cytoskeletal Proteins / physiology*
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Fibroblasts
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Lactams, Macrocyclic
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Methionine / metabolism
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Oncogene Protein pp60(v-src) / metabolism*
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Phosphates / metabolism
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Phosphorylation
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Protein-Tyrosine Kinases / antagonists & inhibitors
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Protein-Tyrosine Kinases / metabolism*
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Quinones / pharmacology
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Rifabutin / analogs & derivatives
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Tyrosine*
Substances
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Benzoquinones
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Cadherins
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Cytoskeletal Proteins
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Lactams, Macrocyclic
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Phosphates
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Quinones
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Rifabutin
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Tyrosine
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herbimycin
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Methionine
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Protein-Tyrosine Kinases
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Oncogene Protein pp60(v-src)