Abstract
Mutations in the human APC gene are linked to familial adenomatous polyposis and to the progression of sporadic colorectal and gastric tumors. To gain insight into APC function, APC-associated proteins were identified by immunoprecipitation experiments. Antibodies to APC precipitated a 95-kilodalton protein that was purified and identified by sequencing as beta-catenin, a protein that binds to the cell adhesion molecule E-cadherin. An antibody specific to beta-catenin also recognized the 95-kilodalton protein in the immunoprecipitates. These results suggest that APC is involved in cell adhesion.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenomatous Polyposis Coli Protein
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Amino Acid Sequence
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Antibodies
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Cadherins / metabolism*
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Cell Adhesion
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Cell Line
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Colonic Neoplasms / genetics
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Colonic Neoplasms / metabolism*
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Cytoskeletal Proteins / chemistry
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Cytoskeletal Proteins / isolation & purification
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Cytoskeletal Proteins / metabolism*
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Genes, APC*
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Humans
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Molecular Sequence Data
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Neoplasm Proteins / genetics
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Neoplasm Proteins / immunology
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Neoplasm Proteins / metabolism*
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Precipitin Tests
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Trans-Activators*
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Tumor Cells, Cultured
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beta Catenin
Substances
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Adenomatous Polyposis Coli Protein
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Antibodies
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CTNNB1 protein, human
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Cadherins
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Cytoskeletal Proteins
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Neoplasm Proteins
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Trans-Activators
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beta Catenin