Abstract
As part of our efforts to understand transcriptional regulation in the vertebrate eye lens, we have isolated a clone that encodes a zinc finger (Zf) protein from a newborn mouse lens cDNA library. Corresponding message for this protein is detectable in the lens, liver, heart, kidney, spleen and brain of newborn mice. A longer cDNA containing the complete ORF for the same protein was isolated from an adult mouse testis library. A conceptual translation of the testis cDNA sequence produces a 555-amino-acid (aa) protein that contains nine C-terminal Zf and an N-terminal domain found in a subset of C2H2 Zf, the Krüppel-associated box (KRAB). The aa sequence located between the KRAB domain and the Zf shows an unexpected similarity to human profilaggrin, a protein expressed in differentiating epidermal cells. Sequences that hybridize to this cDNA are detectable in ten other mammalian species.
MeSH terms
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Amino Acid Sequence
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Animals
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Animals, Newborn
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Base Sequence
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Blotting, Southern
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Conserved Sequence
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DNA-Binding Proteins / genetics*
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Filaggrin Proteins
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Gene Library
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Hominidae / genetics*
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Humans
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Intermediate Filament Proteins / genetics*
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Kidney / metabolism
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Kruppel-Like Transcription Factors
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Lens, Crystalline / metabolism*
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Liver / metabolism
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Male
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Mammals
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Mice / genetics*
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Molecular Sequence Data
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Myocardium / metabolism
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Open Reading Frames
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Organ Specificity
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Phosphoproteins / genetics
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Polymerase Chain Reaction
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Protein Precursors / genetics*
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Repetitive Sequences, Nucleic Acid
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Repressor Proteins*
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Sequence Homology, Amino Acid
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Spleen / metabolism
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Testis / metabolism
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Transcription Factors / genetics*
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Zinc Fingers / genetics*
Substances
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DNA-Binding Proteins
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Filaggrin Proteins
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Intermediate Filament Proteins
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Kruppel-Like Transcription Factors
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Phosphoproteins
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Protein Precursors
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Repressor Proteins
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Transcription Factors