Abstract
We have cloned cDNAs involved in germ cell-specific expression. For this, a subtracted cDNA library was generated by subtracting cDNAs derived from supporting cells of mutant testis from wild-type testis cDNAs. Detailed analyses of mRNA expression revealed that the genes corresponding to the cloned cDNAs were exclusively expressed in testes and were developmentally controlled.
MeSH terms
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Actin Depolymerizing Factors
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Amino Acid Sequence
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Animals
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Base Sequence
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CapZ Actin Capping Protein
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Cell Differentiation
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Cloning, Molecular*
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DNA, Complementary / genetics
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Destrin
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Gene Expression Regulation, Developmental*
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Gene Library
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Male
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Mice
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Microfilament Proteins / chemistry
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Microfilament Proteins / genetics
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Molecular Sequence Data
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Nucleic Acid Hybridization
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RNA Probes
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Spermatids / metabolism*
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Spermatocytes / metabolism
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Spermatogenesis / genetics*
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Spermatogonia / metabolism
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Testicular Hormones / chemistry
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Testicular Hormones / genetics*
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Testis / cytology
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Testis / metabolism*
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Transcription, Genetic
Substances
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Actin Depolymerizing Factors
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CAPZA3 protein, mouse
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CapZ Actin Capping Protein
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DNA, Complementary
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Destrin
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Microfilament Proteins
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RNA Probes
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Testicular Hormones