Abstract
Lambda ZAP cDNA library constructed from spleen of a p53-deficient mouse was screened by South-Western technique using Fragment A, a DNA sequence that p53 specifically binds to, as a probe. One (clone 2) of six clones isolated was identical to MEF2c, a MADS-family transcription factor. Transcripts of the mef2c gene was also detected in spleen where the expression has not been reported so far. Isolated clones except clone 2 had a growth-suppression activity on p53-deficient osteosarcoma cell line (Saos II). Clone 2 repressed the transactivation from Fragment A by p53, suggesting that MEF2c may act as a negative regulator of p53-responsive element.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cell Line
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Consensus Sequence
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DNA, Complementary
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / metabolism*
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Gene Expression
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Gene Library
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Genes, p53
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Humans
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Immunoblotting
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MADS Domain Proteins
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MEF2 Transcription Factors
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Mice
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Mice, Mutant Strains
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Molecular Sequence Data
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Myogenic Regulatory Factors*
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Oligonucleotide Probes
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Spleen / metabolism
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Transcription Factors / biosynthesis
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Transcription Factors / metabolism*
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Transcription, Genetic
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Transcriptional Activation*
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Transfection
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Tumor Suppressor Protein p53 / deficiency
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism*
Substances
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DNA, Complementary
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DNA-Binding Proteins
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MADS Domain Proteins
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MEF2 Transcription Factors
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MEF2C protein, human
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Mef2c protein, mouse
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Myogenic Regulatory Factors
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Oligonucleotide Probes
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Recombinant Proteins
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Transcription Factors
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Tumor Suppressor Protein p53