Cloning of the murine cDNA encoding VDJP, a protein homologous to the large subunit of replication factor C and bacterial DNA ligases

Gene. 1995 Aug 19;161(2):217-22. doi: 10.1016/0378-1119(95)00299-l.

Abstract

A putative full-length 1.7-kb cDNA, encoding a murine protein that specifically binds to the nonamer portion of the V(D)J recombinational signal sequence (RSS) element, has been cloned. By its sequence analysis, this cDNA is identical to a portion of the 4.5-kb murine replication factor C large-subunit-encoding cDNA. By Northern blot analysis, the 1.7-kb mRNA species is observed in murine immature B cells but not in non-lymphoid cells and tissues, while the 4.5-kb replication factor C-encoding cDNA is expressed in all cell types. The deduced VDJP amino-acid sequence includes a region of homology with bacterial DNA ligases at the C terminus of each of the proteins. VDJP has been synthesized as a fusion protein in bacteria, and the purified protein has been previously shown to mediate the joining of DNA fragments in a V(D)J RSS-dependent fashion (Guilliams et al., Biochem. Biophys. Res. Commun. 202 (1994) 1134-1141).

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • DNA Ligases / genetics*
  • DNA Nucleotidyltransferases / biosynthesis
  • DNA Nucleotidyltransferases / genetics*
  • DNA Replication
  • DNA, Complementary / genetics*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • Escherichia coli / genetics
  • Homeodomain Proteins*
  • Mice
  • Minor Histocompatibility Antigens
  • Molecular Sequence Data
  • Mutation
  • Protein Conformation
  • Proto-Oncogene Proteins c-bcl-2*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Replication Protein C
  • Repressor Proteins*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Nucleic Acid
  • VDJ Recombinases

Substances

  • BCL2-related protein A1
  • DNA, Complementary
  • DNA-Binding Proteins
  • Homeodomain Proteins
  • MATA1 protein, S cerevisiae
  • Minor Histocompatibility Antigens
  • Proto-Oncogene Proteins c-bcl-2
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • DNA Nucleotidyltransferases
  • VDJ Recombinases
  • Replication Protein C
  • DNA Ligases

Associated data

  • GENBANK/L17340
  • GENBANK/L23320
  • GENBANK/M88489
  • GENBANK/P15042
  • GENBANK/P26996
  • GENBANK/P28719
  • GENBANK/U01222
  • GENBANK/U03102