Abstract
A putative full-length 1.7-kb cDNA, encoding a murine protein that specifically binds to the nonamer portion of the V(D)J recombinational signal sequence (RSS) element, has been cloned. By its sequence analysis, this cDNA is identical to a portion of the 4.5-kb murine replication factor C large-subunit-encoding cDNA. By Northern blot analysis, the 1.7-kb mRNA species is observed in murine immature B cells but not in non-lymphoid cells and tissues, while the 4.5-kb replication factor C-encoding cDNA is expressed in all cell types. The deduced VDJP amino-acid sequence includes a region of homology with bacterial DNA ligases at the C terminus of each of the proteins. VDJP has been synthesized as a fusion protein in bacteria, and the purified protein has been previously shown to mediate the joining of DNA fragments in a V(D)J RSS-dependent fashion (Guilliams et al., Biochem. Biophys. Res. Commun. 202 (1994) 1134-1141).
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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DNA Ligases / genetics*
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DNA Nucleotidyltransferases / biosynthesis
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DNA Nucleotidyltransferases / genetics*
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DNA Replication
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DNA, Complementary / genetics*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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Escherichia coli / genetics
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Homeodomain Proteins*
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Mice
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Minor Histocompatibility Antigens
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Molecular Sequence Data
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Mutation
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Protein Conformation
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Proto-Oncogene Proteins c-bcl-2*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Replication Protein C
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Repressor Proteins*
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Nucleic Acid
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VDJ Recombinases
Substances
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BCL2-related protein A1
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DNA, Complementary
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DNA-Binding Proteins
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Homeodomain Proteins
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MATA1 protein, S cerevisiae
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Minor Histocompatibility Antigens
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Proto-Oncogene Proteins c-bcl-2
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Recombinant Fusion Proteins
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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DNA Nucleotidyltransferases
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VDJ Recombinases
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Replication Protein C
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DNA Ligases
Associated data
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GENBANK/L17340
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GENBANK/L23320
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GENBANK/M88489
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GENBANK/P15042
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GENBANK/P26996
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GENBANK/P28719
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GENBANK/U01222
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GENBANK/U03102