A cDNA clone from the sea lamprey Petromyzon marinus coding for a scavenger receptor Cys-rich (SRCR) domain protein

Gene. 1995 Oct 27;164(2):267-71. doi: 10.1016/0378-1119(95)94092-z.

Abstract

Our knowledge of the immune system in the early vertebrates, the Agnatha, and the molecules involved in their immune reactions is fragmentary. By serendipity we discovered a cDNA clone in a library made from gut poly(A)+RNA of the sea lamprey, Petromyzon marinus (Pema), that translates into the SREG (SRCR-EGF, see below) protein which resembles cell-membrane proteins of mammalian immune cells. The putative translated product is a type-I integral membrane glycoprotein which contains two scavenger receptor Cys-rich (SRCR) domains flanking five epidermal growth factor (EGF)-like repeats. The two SRCR domains are closely related to CD6 (expressed on human lymphocytes), WC1 (expressed on mammalian CD4-CD8(-)-gamma delta T cells) and M130 (expressed on human macrophages). The Pema-SREG may therefore be involved in intercellular contacts and cell activation or differentiation in the immune system. It is thus a potential marker that can be used to investigate the lamprey immune system.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / biosynthesis
  • Antigens, CD / chemistry
  • Antigens, Differentiation, T-Lymphocyte / biosynthesis
  • Antigens, Differentiation, T-Lymphocyte / chemistry
  • Base Sequence
  • Cell Membrane / immunology
  • Cloning, Molecular
  • Consensus Sequence
  • Cysteine
  • DNA, Complementary / metabolism
  • Epidermal Growth Factor / chemistry
  • Gene Library
  • Humans
  • Lampreys / genetics*
  • Macrophages / immunology
  • Mammals
  • Membrane Glycoproteins / biosynthesis
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics
  • Membrane Proteins*
  • Molecular Sequence Data
  • Phylogeny
  • Protein Biosynthesis
  • Receptors, Immunologic / biosynthesis*
  • Receptors, Immunologic / chemistry
  • Receptors, Immunologic / genetics*
  • Receptors, Lipoprotein*
  • Receptors, Scavenger
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Scavenger Receptors, Class B
  • Sequence Homology, Amino Acid
  • T-Lymphocytes / immunology

Substances

  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CD6 antigen
  • DNA, Complementary
  • Membrane Glycoproteins
  • Membrane Proteins
  • Receptors, Immunologic
  • Receptors, Lipoprotein
  • Receptors, Scavenger
  • Recombinant Proteins
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class B
  • Epidermal Growth Factor
  • Cysteine

Associated data

  • GENBANK/U20652