Analysis of Neisseria gonorrhoeae peptidoglycan by reverse-phase, high-pressure liquid chromatography

J Bacteriol. 1985 Jul;163(1):69-74. doi: 10.1128/jb.163.1.69-74.1985.

Abstract

The muramidase digest of peptidoglycan from Neisseria gonorrhoeae was isolated and analyzed by the use of a reverse-phase, high-pressure liquid chromatography system. As was found previously in the case of Escherichia coli, gonococci peptidoglycan is also composed of a greater number of muropeptides than can be resolved with thin-layer chromatography systems. Preliminary classification of the muropeptide components into subclasses based on O-acetyl modification and degree of cross-linkage was achieved. Examination of a penicillin-susceptible strain and a highly resistant strain with two penicillin-binding protein alterations synthesized distinctly different peptidoglycan structures, as revealed by this technique.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins*
  • Carrier Proteins / genetics
  • Chromatography, High Pressure Liquid
  • Hexosyltransferases*
  • Muramoylpentapeptide Carboxypeptidase / genetics
  • Mutation
  • Neisseria gonorrhoeae / analysis*
  • Neisseria gonorrhoeae / genetics
  • Penicillin Resistance
  • Penicillin-Binding Proteins
  • Peptidoglycan / analysis*
  • Peptidyl Transferases*

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • Penicillin-Binding Proteins
  • Peptidoglycan
  • Peptidyl Transferases
  • Hexosyltransferases
  • Muramoylpentapeptide Carboxypeptidase