Ninein promotes F-actin cup formation and inward phagosome movement during phagocytosis in macrophages

Mol Biol Cell. 2024 Mar 1;35(3):ar26. doi: 10.1091/mbc.E23-06-0216. Epub 2023 Dec 20.

Abstract

Phagocytosis by macrophages is a highly polarized process to destroy large target cells. Binding to particles induces extensive cortical actin-generated forces that drive the formation of elaborate pseudopods around the target particle. Postinternalization, the resultant phagosome is driven toward the cell interior on microtubules (MTs) by cytoplasmic dynein. However, it is unclear whether dynein and cargo-adaptors contribute to the earlier steps of particle internalization and phagosome formation. Here we reveal that ninein, a MT minus-end-associated protein that localizes to the centrosome, is also present at the phagocytic cup in macrophages. Ninein depletion impairs particle internalization by delaying the early F-actin recruitment to sites of particle engagement and cup formation, with no impact on F-actin dynamics beyond this initial step. Ninein forms membrane-bound clusters on phagocytic cups that do not nucleate acentrosomal MTs but instead mediate the assembly of dynein-dynactin complex at active phagocytic membranes. Both ninein depletion and pharmacological inhibition of dynein activity reduced inward displacement of bound particles into macrophages. We found that ninein and dynein motor activity were required for timely retrograde movement of phagosomes and for phagolysosome formation. Taken together, these data show that ninein, alone and with dynein, play significant roles during phagocytosis.

MeSH terms

  • Actins* / metabolism
  • Carrier Proteins / metabolism
  • Cytoskeletal Proteins* / metabolism
  • Dyneins / metabolism
  • Humans
  • Macrophages / metabolism
  • Nuclear Proteins / metabolism
  • Phagocytosis* / physiology
  • Phagosomes / metabolism

Substances

  • Actins
  • Carrier Proteins
  • Dyneins
  • NIN protein, human
  • Nuclear Proteins
  • Cytoskeletal Proteins