Generation of a transgenic mouse embryonic stem cell line overexpressing DNMT1

Sumana Choudhury; Anuhya Anne; Purnima P Pradhan; K Naga Mohan

doi:10.1016/j.scr.2023.103141

Generation of a transgenic mouse embryonic stem cell line overexpressing DNMT1

Stem Cell Res. 2023 Sep:71:103141. doi: 10.1016/j.scr.2023.103141. Epub 2023 Jun 10.

Authors

Sumana Choudhury¹, Anuhya Anne¹, Purnima P Pradhan², K Naga Mohan³

Affiliations

¹ Molecular Biology and Genetics Laboratory, Department of Biological Sciences, BITS Pilani Hyderabad Campus, Hyderabad, India; Centre for Human Disease Research, BITS Pilani Hyderabad Campus, Hyderabad, India.
² Molecular Biology and Genetics Laboratory, Department of Biological Sciences, BITS Pilani Hyderabad Campus, Hyderabad, India.
³ Molecular Biology and Genetics Laboratory, Department of Biological Sciences, BITS Pilani Hyderabad Campus, Hyderabad, India; Centre for Human Disease Research, BITS Pilani Hyderabad Campus, Hyderabad, India. Electronic address: MohanKN@hyderabad.bits-pilani.ac.in.

PMID: 37320987
DOI: 10.1016/j.scr.2023.103141

Abstract

DNMT1 overexpression is reported in disorders like schizophrenia, bipolar, epilepsy and multiple cancer types. Here, we used non-homologous recombination to generate R1^Dnmt1WT-1, a mouse embryonic stem cell (ESC) line carrying a Dnmt1 cDNA transgene to achieve about two-fold overexpression. This ESC line showed increased transcript levels of Sox2 pluripotency marker. R1^Dnmt1WT-1 embryoid bodies showed increased levels of Lefty1 (endoderm), Tbxt and Acta2 (mesoderm), and Pax6 (ectoderm) transcripts. This new line showed normal karyotype and microsatellite profiles making it useful in studying carcinogenesis and abnormal neurogenesis due to DNMT1 overexpression.

Keywords: Cancers; DNMT1 overexpression; Schizophrenia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation
Cell Line
Embryoid Bodies* / metabolism
Endoderm / metabolism
Mice
Mice, Transgenic
Mouse Embryonic Stem Cells* / metabolism