LncRNA FIRRE promotes the proliferation and metastasis of hepatocellular carcinoma by regulating the expression of PXN through interacting with MBNL3

Biochem Biophys Res Commun. 2022 Oct 15:625:188-195. doi: 10.1016/j.bbrc.2022.07.099. Epub 2022 Jul 31.

Abstract

Hepatocellular carcinoma (HCC) has been a long-time public health problem impacting people's heath and challenging healthcare professions because of its poor prognosis and high lethality. More and more evidence indicated the important role of long non-coding RNAs (lncRNAs) in carcinogenesis and cancer metabolism in a variety of cancer types. In this study, we found that FIRRE, a recently identified cancer-associated lncRNA located on chromosome X, is highly expressed in HCC cell lines and tissue samples, and its expression is positively correlated with poor HCC prognosis. In vitro and in vivo functional analyses showed that FIRRE could promote the proliferation, migration, and invasion of HCC. As for the potential mechanism, FIRRE specifically binds to the splicing factor MBNL3 to affect the expression of PXN to regulate the pathological characteristics of HCC cells. In summary, our study showed that the lncRNA FIRRE is a cancer promoting factor and may be a potential biomarker for the prognosis and drug target for the treatment of HCC.

Keywords: FIRRE; HCC; MBNL3; PXN; lncRNA.

MeSH terms

  • Carcinoma, Hepatocellular* / pathology
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Liver Neoplasms* / pathology
  • Paxillin / metabolism
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Up-Regulation

Substances

  • MBNL3 protein, human
  • PXN protein, human
  • Paxillin
  • RNA, Long Noncoding
  • RNA-Binding Proteins