Signal-transducing adaptor protein-2 has a nonredundant role for IL-33-triggered mast cell activation

Biochem Biophys Res Commun. 2021 Oct 1:572:80-85. doi: 10.1016/j.bbrc.2021.07.098. Epub 2021 Jul 31.

Abstract

Signal-transducing adaptor protein (STAP)-2 is one of the STAP family adaptor proteins and ubiquitously expressed in a variety types of cells. Although STAP-2 is required for modification of FcεRI signal transduction in mast cells, other involvement of STAP-2 in mast cell functions is unknown, yet. In the present study, we mainly investigated functional roles of STAP-2 in IL-33-induced mast cell activation. In STAP-2-deficient, but not STAP-1-deficient, mast cells, IL-33-induced IL-6 and TNF-α production was significantly decreased compared with that of wild-type mast cells. In addition, STAP-2-deficiency greatly reduced TLR4-mediated mast cell activation and cytokine production. For the mechanisms, STAP-2 directly binds to IKKα after IL-33 stimulation, leading to elevated NF-κB activity. In conclusion, STAP-2, but not STAP-1, participates in IL-33-induced mast cells activation.

Keywords: IL-33; Mast cells; NF-κB; ST2; Signal-transducing adaptor molecule-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / deficiency
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Animals
  • Cells, Cultured
  • Cytokines / biosynthesis
  • Interleukin-33 / metabolism*
  • Mast Cells / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout

Substances

  • Adaptor Proteins, Signal Transducing
  • Cytokines
  • Il33 protein, mouse
  • Interleukin-33
  • STAP2 protein, mouse