Abstract
We studied metastasis-promoting effect of transmembrane protease TMPRSS4 on mismatch repair (MMR)-deficient colorectal cancer liver metastasis in BALB/c nude mouse model. Histomorphological and histopathological studies showed that the number of liver metastases in the study group were significantly higher than that in the control group (p<0.05). The expression of TMPRSS4 mRNA and protein in the study group were obviously higher than in the control group (p<0.05). These findings suggest that TMPRSS4 possesses a metastasis-promoting effect and its low expression can effectively block the progression of MMR-deficient colon cancer liver metastasis.
Keywords:
BALB/c nude mouse; DNA mismatch repair deficiencies; TMPRSS4; colon cancer liver metastasis; metastasis-promoting effect.
MeSH terms
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Animals
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Brain Neoplasms / complications
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Brain Neoplasms / genetics
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Brain Neoplasms / pathology
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Cell Movement / genetics
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Cell Transformation, Neoplastic / genetics
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Colonic Neoplasms / genetics
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Colonic Neoplasms / pathology*
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Colorectal Neoplasms / complications
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Colorectal Neoplasms / genetics
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Colorectal Neoplasms / pathology
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DNA Mismatch Repair / genetics
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Disease Progression
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Liver Neoplasms / genetics
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Liver Neoplasms / secondary*
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Membrane Proteins / genetics
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Membrane Proteins / physiology*
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplastic Syndromes, Hereditary / complications
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Neoplastic Syndromes, Hereditary / genetics
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Neoplastic Syndromes, Hereditary / pathology
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Serine Endopeptidases / genetics
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Serine Endopeptidases / physiology*
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Tumor Cells, Cultured
Substances
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Membrane Proteins
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Serine Endopeptidases
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TMPRSS4 protein, human
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Tmprss4 protein, mouse