PES1 is regulated by CD44 in liver cancer stem cells via miR-105-5p

FEBS Lett. 2019 Jul;593(14):1777-1786. doi: 10.1002/1873-3468.13459. Epub 2019 Jun 17.

Abstract

Pescadillo (PES1) is a key molecule for ribosome formation in mammalian cells. In this study, human hepatoma C3A cells were reprogrammed by four transcription factors, Oct4, Sox2, Klf4 and c-Myc, into induced cancer stem cells, termed C3A-induced cancer stem cells (C3A-iCSCs). We found that PES1 was up-regulated in C3A-iCSCs and promoted cell proliferation. Moreover, the cancer stem cell marker CD44, which is located in the cytomembrane, translocated to the nucleus and was up-regulated in C3A-iCSCs. Our results suggest that CD44 has a negative effect on miR-105-5p. We found that PES1 is a direct target of, and was negatively regulated by, miR-105-5p. In summary, CD44 regulates PES1 in liver cancer stem cells via miR-105-5p to promote cell growth.

Keywords: C3A cells; CD44; PES1; induced cancer stem cells; miR-105-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Cell Proliferation
  • Hep G2 Cells
  • Humans
  • Hyaluronan Receptors / metabolism*
  • Kruppel-Like Factor 4
  • Liver Neoplasms / pathology*
  • MicroRNAs / genetics*
  • Neoplastic Stem Cells / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Up-Regulation

Substances

  • 3' Untranslated Regions
  • Hyaluronan Receptors
  • KLF4 protein, human
  • Kruppel-Like Factor 4
  • MIRN105 microRNA, human
  • MicroRNAs
  • PES1 protein, human
  • RNA-Binding Proteins