Long non-coding RNA UCA1/miR-182/PFKFB2 axis modulates glioblastoma-associated stromal cells-mediated glycolysis and invasion of glioma cells

Zongze He; Chao You; Dongdong Zhao

doi:10.1016/j.bbrc.2018.04.091

Long non-coding RNA UCA1/miR-182/PFKFB2 axis modulates glioblastoma-associated stromal cells-mediated glycolysis and invasion of glioma cells

Biochem Biophys Res Commun. 2018 Jun 7;500(3):569-576. doi: 10.1016/j.bbrc.2018.04.091. Epub 2018 Apr 25.

Authors

Zongze He¹, Chao You², Dongdong Zhao³

Affiliations

¹ Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu 610041, Sichuan, China; Department of Neurosurgery, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan, China.
² Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu 610041, Sichuan, China. Electronic address: chaoy1955@163.com.
³ Department of Neurosurgery, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan, China. Electronic address: zhaodongdong@med.uestc.edu.cn.

PMID: 29655792
DOI: 10.1016/j.bbrc.2018.04.091

Abstract

Glioblastoma (GB) is the most common and deadliest malignant primary brain tumor with a high recurrence. In this study, lncRNA UCA1/miR-182 axis has been regarded as a nodal driver of glioma invasion mediated by GB-associated stromal cells (GASCs) and GASC-secreted chemokine CXCL14. In clinical specimens, CXCL14 upregulation in GASCs also correlated with poor prognosis. Notably, CXCL14-high GASCs mediated lncRNA UCA1 upregulation and miR-182 downregulation in glioma cells. Moreover, miR-182 directly bound to the fructose-2,6-biphosphatase PFKFB2; UCA1/miR-182 axis thereby modulated GASC-induced glycolysis in glioma cells. Overall, UCA1/miR-182/PFKFB2 axis modulates chemokine CXCL14 secretion, glycolysis and invasion of glioma cells in GASCs.

Keywords: GB-Associated stromal cells (GASCs); Glioblastoma (GB); PFKFB2; lncRNA UCA1; miR-182.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cancer-Associated Fibroblasts / metabolism
Cancer-Associated Fibroblasts / pathology
Cell Line, Tumor
Chemokines, CXC
Gene Expression Regulation, Neoplastic
Glioblastoma / genetics*
Glioblastoma / pathology*
Glycolysis*
Humans
MicroRNAs / genetics
MicroRNAs / metabolism*
Neoplasm Invasiveness
Phenotype
Phosphofructokinase-2 / metabolism*
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Signal Transduction*
Stromal Cells / metabolism
Stromal Cells / pathology
Treatment Outcome

Substances

CXCL14 protein, human
Chemokines, CXC
MicroRNAs
Mirn182 microRNA, human
RNA, Long Noncoding
RNA, Messenger
UCA1 RNA, human
PFKFB2 protein, human
Phosphofructokinase-2