CRIM1, a newfound cancer-related player, regulates the adhesion and migration of lung cancer cells

Hui Zeng; Yi Zhang; Qian Yi; Yanjiao Wu; Rongxue Wan; Liling Tang

doi:10.3109/08977194.2015.1119132

CRIM1, a newfound cancer-related player, regulates the adhesion and migration of lung cancer cells

Growth Factors. 2015;33(5-6):384-92. doi: 10.3109/08977194.2015.1119132. Epub 2015 Dec 10.

Authors

Hui Zeng¹, Yi Zhang¹, Qian Yi¹, Yanjiao Wu¹, Rongxue Wan¹, Liling Tang¹

Affiliation

¹ a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , China.

PMID: 26653968
DOI: 10.3109/08977194.2015.1119132

Abstract

CRIM1 is a member of the bone morphogenetic protein (BMP) antagonists; however, the role of CRIM1 in controlling cancer cell behavior remains unknown. This study investigated its function in the A549 cell line in vitro. The results show that treating cells with CRIM1 peptide could increase the migration and adhesion of A549. Consistently, silencing the CRIM1 expression decreased the migration and adhesion of A549. Furthermore, the CRIM1 protein expression was increased in A549 which were treated with transforming growth factor beta 1 to induced EMT. However, CRIM1 peptide treatment could increase the expression of N-CAD and E-CAD expression. Finally, overexpression of the oncogene YAP1 resulted in an up-regulation of the CRIM1 expression in A549, suggesting that CRIM1 was a target of the Hippo pathway. These observations provide evidence for the first time that CRIM1 plays a role in cancer cells by enhancing the migration and adhesion and increasing the expression of N-CAD and E-CAD.

Keywords: CRIM1; YAP1; adhesion; cancer; migration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / biosynthesis
Antigens, CD / biosynthesis
Bone Morphogenetic Protein Receptors
Bone Morphogenetic Proteins / antagonists & inhibitors
Cadherins / biosynthesis
Carcinoma, Non-Small-Cell Lung / genetics
Carcinoma, Non-Small-Cell Lung / pathology*
Cell Adhesion / drug effects
Cell Adhesion / genetics*
Cell Line, Tumor
Cell Movement / drug effects
Cell Movement / genetics*
Humans
Lung Neoplasms / genetics
Lung Neoplasms / pathology*
Membrane Proteins / genetics*
Membrane Proteins / pharmacology
Phosphoproteins / biosynthesis
RNA Interference
RNA, Small Interfering / genetics
Signal Transduction / drug effects
Transcription Factors
YAP-Signaling Proteins

Substances

Adaptor Proteins, Signal Transducing
Antigens, CD
Bone Morphogenetic Proteins
CDH2 protein, human
CRIM1 protein, human
Cadherins
Membrane Proteins
Phosphoproteins
RNA, Small Interfering
Transcription Factors
YAP-Signaling Proteins
YAP1 protein, human
Bone Morphogenetic Protein Receptors