Evaluation of the automated Vitek 2 system for detection of various mechanisms of macrolide and lincosamide resistance in Staphylococcus aureus

Lorenzo Filippin; Sandrine Roisin; Claire Nonhoff; Stien Vandendriessche; Amélie Heinrichs; Olivier Denis

doi:10.1128/JCM.01617-14

Evaluation of the automated Vitek 2 system for detection of various mechanisms of macrolide and lincosamide resistance in Staphylococcus aureus

J Clin Microbiol. 2014 Nov;52(11):4087-9. doi: 10.1128/JCM.01617-14. Epub 2014 Sep 10.

Authors

Lorenzo Filippin¹, Sandrine Roisin², Claire Nonhoff¹, Stien Vandendriessche¹, Amélie Heinrichs¹, Olivier Denis¹

Affiliations

¹ National Reference Centre-Staphylococcus aureus, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.
² National Reference Centre-Staphylococcus aureus, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium sandrine.roisin@erasme.ulb.ac.be.

Abstract

We evaluated the performance of the automated Vitek 2 system against disk diffusion for susceptibility testing of Staphylococcus aureus strains showing various resistance mechanisms to macrolides and lincosamides (ML). The Vitek 2 system showed 100% concordance with the D-zone test in detection of the most common resistance mechanisms to ML, including methylase and efflux systems.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Automation, Laboratory / methods*
Humans
Lincosamides / pharmacology*
Macrolides / pharmacology*
Microbial Sensitivity Tests / methods*
Phenotype
Staphylococcus aureus / drug effects*
Staphylococcus aureus / growth & development

Substances

Anti-Bacterial Agents
Lincosamides
Macrolides