MicroRNA targeting of CoREST controls polarization of migrating cortical neurons

Marie-Laure Volvert; Pierre-Paul Prévot; Pierre Close; Sophie Laguesse; Sophie Pirotte; James Hemphill; Florence Rogister; Nathalie Kruzy; Rosalie Sacheli; Gustave Moonen; Alexander Deiters; Matthias Merkenschlager; Alain Chariot; Brigitte Malgrange; Juliette D Godin; Laurent Nguyen

doi:10.1016/j.celrep.2014.03.075

MicroRNA targeting of CoREST controls polarization of migrating cortical neurons

Cell Rep. 2014 May 22;7(4):1168-83. doi: 10.1016/j.celrep.2014.03.075. Epub 2014 May 1.

Authors

Marie-Laure Volvert¹, Pierre-Paul Prévot¹, Pierre Close², Sophie Laguesse¹, Sophie Pirotte¹, James Hemphill³, Florence Rogister¹, Nathalie Kruzy¹, Rosalie Sacheli¹, Gustave Moonen¹, Alexander Deiters³, Matthias Merkenschlager⁴, Alain Chariot⁵, Brigitte Malgrange¹, Juliette D Godin¹, Laurent Nguyen⁶

Affiliations

¹ GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
² GIGA-Signal Transduction, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
³ Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
⁴ Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK.
⁵ GIGA-Signal Transduction, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Walloon Excellence in Lifesciences and Biotechnology (WELBIO), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium.
⁶ GIGA-Neurosciences, University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium; Walloon Excellence in Lifesciences and Biotechnology (WELBIO), University of Liège, C.H.U. Sart Tilman, Liège 4000, Belgium. Electronic address: lnguyen@ulg.ac.be.

PMID: 24794437
DOI: 10.1016/j.celrep.2014.03.075

Abstract

The migration of cortical projection neurons is a multistep process characterized by dynamic cell shape remodeling. The molecular basis of these changes remains elusive, and the present work describes how microRNAs (miRNAs) control neuronal polarization during radial migration. We show that miR-22 and miR-124 are expressed in the cortical wall where they target components of the CoREST/REST transcriptional repressor complex, thereby regulating doublecortin transcription in migrating neurons. This molecular pathway underlies radial migration by promoting dynamic multipolar-bipolar cell conversion at early phases of migration, and later stabilization of cell polarity to support locomotion on radial glia fibers. Thus, our work emphasizes key roles of some miRNAs that control radial migration during cerebral corticogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Movement / physiology*
Cell Polarity / physiology
Cells, Cultured
Cerebral Cortex / cytology*
Co-Repressor Proteins
Mice
Mice, Transgenic
MicroRNAs / metabolism*
Nerve Tissue Proteins / metabolism*
Neurons / cytology*
Neurons / metabolism
Repressor Proteins / metabolism*

Substances

Co-Repressor Proteins
MicroRNAs
Mirn124 microRNA, mouse
Mirn22 microRNA, mouse
Nerve Tissue Proteins
RE1-silencing transcription factor
Rcor2 protein, mouse
Repressor Proteins

Grants and funding

MC_U120027516/MRC_/Medical Research Council/United Kingdom