Reciprocal effects between microRNA-140-5p and ADAM10 suppress migration and invasion of human tongue cancer cells

Yang Kai; Wang Peng; Wu Ling; Hao Jiebing; Bian Zhuan

doi:10.1016/j.bbrc.2014.02.032

Reciprocal effects between microRNA-140-5p and ADAM10 suppress migration and invasion of human tongue cancer cells

Biochem Biophys Res Commun. 2014 Jun 6;448(3):308-14. doi: 10.1016/j.bbrc.2014.02.032. Epub 2014 Feb 13.

Authors

Yang Kai¹, Wang Peng¹, Wu Ling¹, Hao Jiebing², Bian Zhuan³

Affiliations

¹ State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
² The Second Charity Hospital of Henan Province, Jiaozuo 454000, China. Electronic address: haojiebing@126.com.
³ State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address: BZ@whuss.com.

PMID: 24530397
DOI: 10.1016/j.bbrc.2014.02.032

Abstract

ADAM10, overexpressed in tongue squamous cell carcinoma (TSCC), has been well documented for its role in tumor progression and metastasis. In the present study, we evaluated the inhibition effect of microRNAs (miRNAs) on the TSCC and identified that miR-140-5p could directly targets ADAM10 and inhibits the invasion and migration of TSCC cells. LAMC1, HDAC7 and PAX6, clustered into migration-related genes, were validated to be direct targets of miR-140-5p, while IGF1R and PSEN1 were not responsible to the regulation. Most intriguingly, ERBB4 was upregulated by miR-140-5p even though the interaction between ERBB4 3'UTR and miR-140-5p existed simultaneously. Meanwhile, ADAM10 is involved in the "positive" regulation of ERBB4 and negative regulation of PAX6 by miR-140-5p. Taken together, our results suggest that miR-140-5p play a role in TSCC cell migration and invasion, and two brand new relationships between miRNA and its targets emerged: (1) ADAM10 is not just a direct target of miR-140-5p, the repressed ADAM10 also helps to enhance the effect of miR-140-5p to other target genes: ERBB4 and PAX6; (2) ERBB4 is "positively" regulated by miR-140-5p.

Keywords: ADAM10; ERBB4; MicroRNA; Tongue squamous cell carcinoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
ADAM Proteins / antagonists & inhibitors
ADAM Proteins / genetics*
ADAM Proteins / metabolism
ADAM10 Protein
Amyloid Precursor Protein Secretases / antagonists & inhibitors
Amyloid Precursor Protein Secretases / genetics*
Amyloid Precursor Protein Secretases / metabolism
Base Sequence
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / metabolism
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation
ErbB Receptors / genetics
Eye Proteins / genetics
Gene Expression Regulation, Neoplastic
Genes, Tumor Suppressor
Homeodomain Proteins / genetics
Humans
Membrane Proteins / antagonists & inhibitors
Membrane Proteins / genetics*
Membrane Proteins / metabolism
MicroRNAs / genetics*
MicroRNAs / metabolism
Neoplasm Invasiveness / genetics
Neoplasm Invasiveness / pathology
Neoplasm Invasiveness / prevention & control
PAX6 Transcription Factor
Paired Box Transcription Factors / genetics
RNA, Neoplasm / genetics
RNA, Neoplasm / metabolism
RNA, Small Interfering / genetics
Receptor, ErbB-4
Repressor Proteins / genetics
Tongue Neoplasms / genetics*
Tongue Neoplasms / metabolism
Tongue Neoplasms / pathology*

Substances

3' Untranslated Regions
Eye Proteins
Homeodomain Proteins
Membrane Proteins
MicroRNAs
Mirn140 microRNA, human
PAX6 Transcription Factor
PAX6 protein, human
Paired Box Transcription Factors
RNA, Neoplasm
RNA, Small Interfering
Repressor Proteins
ERBB4 protein, human
ErbB Receptors
Receptor, ErbB-4
Amyloid Precursor Protein Secretases
ADAM Proteins
ADAM10 Protein
ADAM10 protein, human