Abstract
Crude fractions of urine from pregnant women are immunosuppressive in vitro. An 85-kilodalton immunosuppressive glycoprotein purified to homogeneity from such urine inhibited in vitro assays of human T-cell and monocyte activity at concentrations of 10(-9) to 10(-11) molar. This material was nontoxic and blocked early events required for normal T-cell proliferation in vitro. On the basis of its tissue source and its in vitro activity, the name "uromodulin" is proposed for this glycoprotein.
MeSH terms
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B-Lymphocytes / drug effects
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Chromatography / methods
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Collodion
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Cytotoxicity, Immunologic / drug effects
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Electrophoresis, Polyacrylamide Gel
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Epitopes
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Female
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Hemolytic Plaque Technique
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Humans
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Immunosuppressive Agents / isolation & purification
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Immunosuppressive Agents / urine*
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In Vitro Techniques
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Isoelectric Focusing
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Lymphocyte Activation / drug effects
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Molecular Weight
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Mucoproteins*
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Pregnancy
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Pregnancy Proteins / isolation & purification
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Pregnancy Proteins / pharmacology
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Pregnancy Proteins / urine*
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T-Lymphocytes / drug effects
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Uromodulin
Substances
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Epitopes
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Immunosuppressive Agents
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Mucoproteins
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Pregnancy Proteins
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UMOD protein, human
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Uromodulin
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Collodion