Abstract
The timing and progression of axonal myelination are precisely controlled by intercellular interactions between neurons and glia in development. Previous in vitro studies demonstrated that Nectin like 4 (Necl-4, also known as cell adhesion molecule Cadm-4 or SynCAM-4) plays an essential role in axonal myelination by Schwann cells in the peripheral nervous system (PNS). However, the role of Necl-4 protein in axonal myelination in the developing central nervous system (CNS) has remained unknown. In this study, we discovered upregulation of Necl-4 expression in mature oligodendrocytes at perinatal stages when axons undergo active myelination. We generated Necl4 gene knockout mice, but found that disruption of Necl-4 gene did not affect oligodendrocyte differentiation and myelin formation in the CNS. Surprisingly, disruption of Necl-4 had no significant effect on axonal myelination in the PNS either. Therefore, our results demonstrated that Necl-4 is dispensable for axonal myelination in the developing nervous system.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Axons / metabolism*
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Basic Helix-Loop-Helix Transcription Factors / genetics
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Basic Helix-Loop-Helix Transcription Factors / metabolism
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Brain / cytology
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Brain / metabolism
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Cell Adhesion Molecule-1
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / metabolism*
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Cell Differentiation
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Gene Expression
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Gene Knockout Techniques
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Homeobox Protein Nkx-2.2
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Immunoglobulins / genetics
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Immunoglobulins / metabolism*
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Mice
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Mice, Knockout
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Myelin Sheath / metabolism*
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Oligodendroglia / metabolism*
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Oligodendroglia / physiology
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Peripheral Nervous System / cytology
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Peripheral Nervous System / metabolism
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Spinal Cord / cytology
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Spinal Cord / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Zebrafish Proteins
Substances
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Basic Helix-Loop-Helix Transcription Factors
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Cadm1 protein, mouse
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Cell Adhesion Molecule-1
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Cell Adhesion Molecules
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Homeobox Protein Nkx-2.2
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Homeodomain Proteins
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Igsf4b protein, mouse
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Igsf4c protein, mouse
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Immunoglobulins
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Olig1 protein, mouse
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Transcription Factors
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Zebrafish Proteins