Mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression

Noelia Fradejas; Bradley A Carlson; Eddy Rijntjes; Niels-Peter Becker; Ryuta Tobe; Ulrich Schweizer

doi:10.1042/BJ20121713

Mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression

Biochem J. 2013 Mar 1;450(2):427-32. doi: 10.1042/BJ20121713.

Authors

Noelia Fradejas¹, Bradley A Carlson, Eddy Rijntjes, Niels-Peter Becker, Ryuta Tobe, Ulrich Schweizer

Affiliation

¹ Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, 13353 Berlin, Germany.

PMID: 23289710
DOI: 10.1042/BJ20121713

Abstract

Selenoproteins are proteins carrying the rare amino acid Sec (selenocysteine). Full expression of selenoproteins requires modification of tRNA([Ser]Sec), including N(6)-isopentenylation of base A(37). We show that Trit1 is a dimethylallyl:tRNA([Ser]Sec) transferase. Knockdown of Trit1 reduces expression of selenoproteins. Incubation of in vitro transcribed tRNA[Ser]Sec with recombinant Trit1 transfers [(14)C]dimethylallyl pyrophosphate to tRNA([Ser]Sec). 37A>G tRNA([Ser]Sec) is resistant to isopentenylation by Trit1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkyl and Aryl Transferases / genetics*
Alkyl and Aryl Transferases / metabolism
Animals
Base Sequence
Hep G2 Cells
Humans
Mice
Molecular Sequence Data
NIH 3T3 Cells
Nucleic Acid Conformation
RNA, Transfer, Amino Acyl / genetics*
RNA, Transfer, Amino Acyl / metabolism
Selenoproteins / genetics*
Selenoproteins / metabolism

Substances

RNA, Transfer, Amino Acyl
Selenoproteins
selenocysteinyl-tRNA
Alkyl and Aryl Transferases
tRNA((Ser)Sec)-isopentenyl transferase, mouse