Nucleotide sequence of cDNA for nuclear encoded subunit Vb of mouse cytochrome-c oxidase

Biochim Biophys Acta. 1990 Sep 10;1087(1):98-100. doi: 10.1016/0167-4781(90)90128-o.

Abstract

We have used a polyclonal antibody probe to isolate cDNA clones for mouse cytochrome oxidase subunit Vb from a bone marrow tumor cell mRNA library in lambda gt11 expression vector. The mouse cDNA contains an open reading frame of 128 amino acids, which shows 81% positional identity with the predicted amino acid sequences of the human subunit. Northern blot analysis and sequencing of cDNA from a mouse kidney library show no tissue specific variations in subunit Vb.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Nucleus / enzymology
  • DNA / analysis
  • Electron Transport Complex IV / genetics*
  • Genetic Variation
  • Genomic Library
  • Humans
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • RNA, Messenger / biosynthesis
  • Tumor Cells, Cultured

Substances

  • RNA, Messenger
  • DNA
  • Electron Transport Complex IV

Associated data

  • GENBANK/X53157