Green tea polyphenol epigallocatechin-3-gallate inhibits TLR2 signaling induced by peptidoglycan through the polyphenol sensing molecule 67-kDa laminin receptor

FEBS Lett. 2011 Mar 9;585(5):814-20. doi: 10.1016/j.febslet.2011.02.010. Epub 2011 Feb 12.

Abstract

Here we show the molecular basis for the inhibition of peptidoglycan (PGN)-induced TLR2 signaling by a major green tea polyphenol epigallocatechin-3-gallate (EGCG). Recently, we identified the 67-kDa laminin receptor (67LR) as the cell-surface EGCG receptor. Anti-67LR antibody treatment or silencing of 67LR resulted in abrogation of the inhibitory action of EGCG on PGN-induced production of pro-inflammatory mediators and activation of mitogen-activated protein kinases. Silencing of Toll-interacting protein (Tollip), a negative regulator of TLR signaling impaired the TLR2 signaling inhibitory activity of EGCG, suggesting that TLR2 response could be inhibited by EGCG via 67LR and Tollip.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology
  • Down-Regulation / drug effects
  • Enzyme Activation / drug effects
  • Flavonoids / pharmacology
  • Inflammation Mediators / metabolism
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • MAP Kinase Signaling System / drug effects
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / metabolism
  • Mice
  • Molecular Weight
  • Peptidoglycan / pharmacology*
  • Phenols / pharmacology
  • Polyphenols
  • Receptors, Laminin / metabolism*
  • Signal Transduction / drug effects*
  • Tea / chemistry*
  • Toll-Like Receptor 2 / metabolism*

Substances

  • Flavonoids
  • Inflammation Mediators
  • Intracellular Signaling Peptides and Proteins
  • Peptidoglycan
  • Phenols
  • Polyphenols
  • Receptors, Laminin
  • Tea
  • Tlr2 protein, mouse
  • Toll-Like Receptor 2
  • Tollip protein, mouse
  • Catechin
  • epigallocatechin gallate