Abstract
Many proteins within eukaryotic cells are organized spatially and functionally into membrane bound organelles and complexes. A protein's location thus provides information about its function. Here, we apply LOPIT, a mass-spectrometry based technique that simultaneously maps proteins to specific subcellular compartments, to Drosophila embryos. We determine the subcellular distribution of hundreds of proteins, and protein complexes. Our results reveal the potential of LOPIT to provide average snapshots of cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Centrifugation, Density Gradient
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Drosophila Proteins / analysis*
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Drosophila Proteins / chemistry
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Drosophila melanogaster / chemistry*
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Drosophila melanogaster / ultrastructure*
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Isotope Labeling
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Microscopy, Fluorescence
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Multiprotein Complexes / analysis*
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Multiprotein Complexes / metabolism
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Organelles / chemistry*
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Peptide Fragments / analysis
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Peptide Fragments / metabolism
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Peptide Mapping / methods*
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Principal Component Analysis
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Proteomics / methods
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Reproducibility of Results
Substances
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Drosophila Proteins
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Multiprotein Complexes
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Peptide Fragments