Abstract
Expression of focal adhesion protein vinexin is reported to be altered in several cancer tissues; however, the mechanism of expressional change in vinexin is not known. Here we report the suppression of vinexin expression according to cellular transformation by v-Src. We found that vinexin expression was down-regulated both at the mRNA level and at the post-transcriptional level in v-Src-transformed cells. Both mTOR and MEK/ERK signals were involved in the suppression. Inhibition of these pathways by pharmacological treatment partially restored both vinexin protein and mRNA expression. Moreover, re-expression of vinexin in v-Src-transformed cells suppressed cell migration. Taken together, these observations suggest that cellular transformation by v-Src suppressed vinexin expression and that down-regulation of vinexin may be associated with oncogenic transformation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carrier Proteins / metabolism
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Cell Movement
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Cell Transformation, Neoplastic / genetics
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Cell Transformation, Neoplastic / metabolism*
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Cell Transformation, Neoplastic / pathology
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Cell Transformation, Viral / genetics*
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Dose-Response Relationship, Drug
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Down-Regulation
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Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Focal Adhesions / drug effects
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Focal Adhesions / metabolism*
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Genes, src*
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Mice
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Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors
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Mitogen-Activated Protein Kinase Kinases / metabolism
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Muscle Proteins / genetics
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Muscle Proteins / metabolism*
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NIH 3T3 Cells
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Protein Kinase Inhibitors / pharmacology
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RNA, Messenger / metabolism
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TOR Serine-Threonine Kinases
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Time Factors
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Transfection
Substances
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Carrier Proteins
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Muscle Proteins
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Protein Kinase Inhibitors
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RNA, Messenger
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Sorbs3 protein, mouse
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Phosphotransferases (Alcohol Group Acceptor)
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mTOR protein, mouse
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TOR Serine-Threonine Kinases
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Extracellular Signal-Regulated MAP Kinases
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Mitogen-Activated Protein Kinase Kinases