Abstract
Eaf2 encodes a tumor suppressor that plays multiple functions in transcriptional activation, apoptosis, and embryonic development. In this study, we utilized GFP-EAF2 fusion protein to describe the dynamic subcellular movement of Eaf2. GFP-EAF2 is preferentially localized to the nucleus, and in the presence of ELL, it accumulates in nuclear speckles. However, Eaf2 is an unstable nuclear protein whose stability is affected by serum. Further, we provided first evidence that nuclear distribution of Eaf2 is responsive to DNA damage. Following UV irradiation, Eaf2 is relocalized to the nucleolus, suggesting a possible functional involvement of Eaf2 in DNA damage response.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Cell Nucleus / metabolism
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Cell Nucleus / radiation effects
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Cells, Cultured
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Cytoplasm / metabolism
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Cytoplasm / radiation effects
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DNA Damage / physiology*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Mice
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Nuclear Proteins / physiology*
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Protein Processing, Post-Translational / radiation effects
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Protein Structure, Tertiary
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Tissue Distribution / radiation effects
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Trans-Activators / chemistry
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Trans-Activators / physiology*
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription Factors / physiology*
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Transfection
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Ultraviolet Rays*
Substances
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FESTA protein, mouse
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Nuclear Proteins
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Recombinant Proteins
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Trans-Activators
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Transcription Factors
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Green Fluorescent Proteins