Abstract
Dendritic cells are key initiators and regulators of the immune response. Dendritic cell commitment and function require orchestrated regulation of transcription. Gata1 is a transcription factor expressed in several hematopoietic lineages. However, Gata1 function has not been explored in the monocytic or dendritic cell compartment. Here, we show that Gata1 is expressed in myeloid and plasmacytoid dendritic cells and that Gata1 ablation affects the survival of dendritic cells. Furthermore, lipopolysaccharide (LPS) stimulation of dendritic cells prompts Gata1 up-regulation, which is accompanied by increased levels of BclX and Ifng. Our findings show that Gata1 is a transcriptional regulator of dendritic cell differentiation and suggest that Gata1 is involved in the dendritic cell and macrophage lineage separation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Cell Differentiation*
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Cell Lineage*
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Cell Survival*
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Cells, Cultured
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Chromatin Immunoprecipitation
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Dendritic Cells / cytology*
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Dendritic Cells / drug effects
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Dendritic Cells / metabolism
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Enzyme-Linked Immunosorbent Assay
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Female
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Flow Cytometry
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Fluorescent Antibody Technique
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GATA1 Transcription Factor / genetics
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GATA1 Transcription Factor / physiology*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Integrases / metabolism
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Interferon-gamma / metabolism
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Lipopolysaccharides / pharmacology
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Macrophages / cytology
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Macrophages / drug effects
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Macrophages / metabolism
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Male
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Mice
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Mice, Knockout
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Myeloid Cells / metabolism
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Promoter Regions, Genetic
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism
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Trans-Activators / genetics
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Trans-Activators / metabolism
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bcl-X Protein / metabolism
Substances
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Bcl2l1 protein, mouse
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GATA1 Transcription Factor
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Gata1 protein, mouse
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Lipopolysaccharides
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Proto-Oncogene Proteins
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Trans-Activators
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bcl-X Protein
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proto-oncogene protein Spi-1
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Green Fluorescent Proteins
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Interferon-gamma
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Cre recombinase
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Integrases