Interactions of ribosomal protein S1 with DsrA and rpoS mRNA

Biochem Biophys Res Commun. 2006 Sep 22;348(2):662-8. doi: 10.1016/j.bbrc.2006.07.102. Epub 2006 Jul 28.

Abstract

Ribosomal protein S1 is shown to interact with the non-coding RNA DsrA and with rpoS mRNA. DsrA is a non-coding RNA that is important in controlling expression of the rpoS gene product in Escherichia coli. Photochemical crosslinking, quadrupole-time of flight tandem mass spectrometry, and peptide sequencing have identified an interaction between DsrA and S1 in the 30S ribosomal subunit. Purified S1 binds both DsrA (K(obs) approximately 6 x 10(6) M(-1)) and rpoS mRNA (K(obs) approximately 3 x 10(7) M(-1)). Ribonuclease probing experiments indicate that S1 binding has a weak but detectable effect on the secondary structure of DsrA or rpoS mRNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Electrophoretic Mobility Shift Assay
  • Escherichia coli Proteins / metabolism*
  • Mass Spectrometry
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • RNA, Messenger / metabolism*
  • RNA, Small Untranslated
  • RNA, Untranslated / metabolism*
  • Ribonuclease, Pancreatic / metabolism
  • Ribosomal Proteins / metabolism*
  • Sigma Factor / genetics*

Substances

  • Bacterial Proteins
  • DsrA RNA, E coli
  • Escherichia coli Proteins
  • Peptide Fragments
  • RNA, Messenger
  • RNA, Small Untranslated
  • RNA, Untranslated
  • Ribosomal Proteins
  • Sigma Factor
  • ribosomal protein S1
  • sigma factor KatF protein, Bacteria
  • Ribonuclease, Pancreatic