Hzf protein regulates dendritic localization and BDNF-induced translation of type 1 inositol 1,4,5-trisphosphate receptor mRNA

Takatoshi Iijima; Takao Imai; Yuki Kimura; Alan Bernstein; Hirotaka James Okano; Michisuke Yuzaki; Hideyuki Okano

doi:10.1073/pnas.0504684102

Hzf protein regulates dendritic localization and BDNF-induced translation of type 1 inositol 1,4,5-trisphosphate receptor mRNA

Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17190-5. doi: 10.1073/pnas.0504684102. Epub 2005 Nov 14.

Authors

Takatoshi Iijima¹, Takao Imai, Yuki Kimura, Alan Bernstein, Hirotaka James Okano, Michisuke Yuzaki, Hideyuki Okano

Affiliation

¹ Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan.

Abstract

The localization of certain mRNAs to dendrites and their local translation in synaptic regions are proposed to be involved in certain aspects of synaptic plasticity. A cis-acting element within the 3' untranslated region (3' UTR) of the targeted mRNAs, which is bound by a trans-acting RNA-binding protein, controls the dendritic mRNA localization. Here, we identified hematopoietic zinc finger (Hzf) as a trans-acting factor that regulates the dendritic mRNA localization of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)RI), a dendritically localized mRNA in cerebellar Purkinje cells, via binding to the 3' UTR. In Hzf-deficient mice, the dendritic localization of IP(3)RI mRNA and brain-derived neurotrophic factor-induced IP(3)RI protein synthesis in the cerebellum were impaired. These findings suggest that Hzf is an RNA-binding protein that controls the dendritic mRNA localization and activity-dependent translation of IP(3)RI, and may be involved in some aspects of synaptic plasticity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions / metabolism
3' Untranslated Regions / physiology
Animals
Brain-Derived Neurotrophic Factor / physiology*
Calcium Channels / genetics*
Calcium Channels / metabolism*
Calcium Channels / physiology
Cells, Cultured
Cerebellum / metabolism
Dendrites / metabolism*
Dendrites / physiology
Inositol 1,4,5-Trisphosphate Receptors
Mice
Neuronal Plasticity / genetics
Neuronal Plasticity / physiology
Proteins / genetics
Proteins / physiology*
Purkinje Cells / metabolism
RNA Processing, Post-Transcriptional / genetics
RNA, Messenger / physiology*
Receptors, Cytoplasmic and Nuclear / genetics*
Receptors, Cytoplasmic and Nuclear / metabolism*
Receptors, Cytoplasmic and Nuclear / physiology
Synapses / physiology
Trans-Activators / metabolism

Substances

3' Untranslated Regions
Brain-Derived Neurotrophic Factor
Calcium Channels
Inositol 1,4,5-Trisphosphate Receptors
Proteins
RNA, Messenger
Receptors, Cytoplasmic and Nuclear
Trans-Activators
Zfp385 protein, mouse