Abstract
We have identified a cDNA coding for a protein of 160 kDa which is expressed in in vitro cultured human peripheral blood monocytes. The predicted amino acid sequence contains an alpha-helical rod domain possessing features characteristic of intermediate filament proteins. However, the immunocytochemical staining pattern, abundance and solubility in Triton X-100/high salt buffers suggest that this protein is probably only associated with the intermediate filament network and represents a new type of intermediate filament associated protein. In a survey of normal, inflammatory and human tumour tissue samples, this protein, which we have named restin, was found to be highly expressed in Reed-Sternberg cells, the tumoral cells diagnostic for Hodgkin's disease. We suggest that restin overexpression may be a contributing factor in the progression of Hodgkin's disease.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
CHO Cells
-
Cell Line
-
Cells, Cultured
-
Cloning, Molecular
-
Cricetinae
-
DNA, Neoplasm / genetics
-
DNA, Neoplasm / isolation & purification
-
Gene Library
-
Hodgkin Disease / genetics*
-
Hodgkin Disease / metabolism
-
Humans
-
Intermediate Filament Proteins / genetics*
-
Intermediate Filament Proteins / isolation & purification
-
Intermediate Filament Proteins / metabolism
-
Microtubule-Associated Proteins*
-
Molecular Sequence Data
-
Monocytes / cytology
-
Monocytes / metabolism
-
Monocytes / pathology
-
Neoplasm Proteins / genetics*
-
Neoplasm Proteins / isolation & purification
-
Neoplasm Proteins / metabolism
-
Protein Conformation
-
Reed-Sternberg Cells / metabolism*
-
Sequence Homology, Nucleic Acid
-
Transfection
-
Vimentin / analysis
Substances
-
DNA, Neoplasm
-
Intermediate Filament Proteins
-
Microtubule-Associated Proteins
-
Neoplasm Proteins
-
Vimentin
-
cytoplasmic linker protein 170
Associated data
-
GENBANK/X64335
-
GENBANK/X64555
-
GENBANK/X64556
-
GENBANK/X64629
-
GENBANK/X64700
-
GENBANK/X64838
-
GENBANK/X67723
-
GENBANK/X67724
-
GENBANK/X67725
-
GENBANK/X67726