Inhibition of HIV-1 TAR RNA-Tat peptide complexation using poly(acrylic acid)

Hong Zhao; Jinru Li; Long Jiang

doi:10.1016/j.bbrc.2004.05.148

Inhibition of HIV-1 TAR RNA-Tat peptide complexation using poly(acrylic acid)

Biochem Biophys Res Commun. 2004 Jul 16;320(1):95-9. doi: 10.1016/j.bbrc.2004.05.148.

Authors

Hong Zhao¹, Jinru Li, Long Jiang

Affiliation

¹ Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.

PMID: 15207707
DOI: 10.1016/j.bbrc.2004.05.148

Abstract

HIV-1 is regulated at the transcriptional level by the interaction of Tat protein with the transactivation responsive region (TAR) RNA, a 59-base stem-loop structure located at the 5'-end of all nascent HIV-1 transcripts. Here, by targeting the Tat peptide, we found that negatively charged poly(acrylic acid) (PAA) had high affinity with Tat peptide and could inhibit the interaction of TAR with Tat. Therefore, PAA could block HIV replication by binding to Tat not to TAR RNA, providing a new thinking for the design of novel anti-HIV drugs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemistry*
Binding Sites
Gene Products, tat / chemistry*
Gene Products, tat / ultrastructure*
HIV Long Terminal Repeat*
HIV-1 / genetics
Macromolecular Substances
Molecular Weight
Nucleic Acid Conformation
Protein Binding
RNA, Viral / chemistry*
RNA, Viral / ultrastructure
tat Gene Products, Human Immunodeficiency Virus

Substances

Acrylic Resins
Gene Products, tat
Macromolecular Substances
RNA, Viral
tat Gene Products, Human Immunodeficiency Virus
carbopol 940