alpha3beta1 integrin-CD151, a component of the cadherin-catenin complex, regulates PTPmu expression and cell-cell adhesion

J Cell Biol. 2003 Dec 22;163(6):1351-62. doi: 10.1083/jcb.200306067.

Abstract

The beta1 family of integrins has been primarily studied as a set of receptors for the extracellular matrix. In this paper, we define a novel role for alpha3beta1 integrin in association with the tetraspanin CD151 as a component of a cell-cell adhesion complex in epithelial cells that directly stimulates cadherin-mediated adhesion. The integrin-tetraspanin complex affects epithelial cell-cell adhesion at the level of gene expression both by regulating expression of PTPmu and by organizing a multimolecular complex containing PKCbetaII, RACK1, PTPmu, beta-catenin, and E-cadherin. These findings demonstrate how integrin-based signaling can regulate complex biological responses at multiple levels to determine cell morphology and behavior.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD / metabolism*
  • Cadherins / metabolism
  • Cell Adhesion / physiology
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism
  • Cell Communication / physiology
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Cytoskeletal Proteins / metabolism
  • Cytoskeleton / metabolism
  • Epithelial Cells / metabolism*
  • GTP-Binding Proteins
  • Integrin alpha3beta1 / deficiency
  • Integrin alpha3beta1 / genetics
  • Integrin alpha3beta1 / metabolism*
  • Laminin / metabolism
  • Macromolecular Substances
  • Mice
  • Neoplasm Proteins / metabolism
  • Protein Kinase C / metabolism
  • Protein Kinase C beta
  • Protein Tyrosine Phosphatases / metabolism*
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2
  • Receptor-Like Protein Tyrosine Phosphatases, Class 8
  • Receptors for Activated C Kinase
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Tetraspanin 24
  • Trans-Activators / metabolism
  • beta Catenin

Substances

  • Antigens, CD
  • CD151 protein, human
  • CTNNB1 protein, mouse
  • Cadherins
  • Cd151 protein, mouse
  • Cell Adhesion Molecules
  • Cytoskeletal Proteins
  • Integrin alpha3beta1
  • Laminin
  • Macromolecular Substances
  • Neoplasm Proteins
  • RACK1 protein, human
  • Receptors for Activated C Kinase
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • Tetraspanin 24
  • Trans-Activators
  • beta Catenin
  • laminin 1
  • Protein Kinase C
  • Protein Kinase C beta
  • PTPRN protein, human
  • Protein Tyrosine Phosphatases
  • Ptprn protein, mouse
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2
  • Receptor-Like Protein Tyrosine Phosphatases, Class 8
  • GTP-Binding Proteins