Abstract
The beta1 family of integrins has been primarily studied as a set of receptors for the extracellular matrix. In this paper, we define a novel role for alpha3beta1 integrin in association with the tetraspanin CD151 as a component of a cell-cell adhesion complex in epithelial cells that directly stimulates cadherin-mediated adhesion. The integrin-tetraspanin complex affects epithelial cell-cell adhesion at the level of gene expression both by regulating expression of PTPmu and by organizing a multimolecular complex containing PKCbetaII, RACK1, PTPmu, beta-catenin, and E-cadherin. These findings demonstrate how integrin-based signaling can regulate complex biological responses at multiple levels to determine cell morphology and behavior.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, CD / metabolism*
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Cadherins / metabolism
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Cell Adhesion / physiology
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / metabolism
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Cell Communication / physiology
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Cell Membrane / metabolism
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Cells, Cultured
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Cytoskeletal Proteins / metabolism
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Cytoskeleton / metabolism
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Epithelial Cells / metabolism*
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GTP-Binding Proteins
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Integrin alpha3beta1 / deficiency
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Integrin alpha3beta1 / genetics
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Integrin alpha3beta1 / metabolism*
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Laminin / metabolism
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Macromolecular Substances
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Mice
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Neoplasm Proteins / metabolism
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Protein Kinase C / metabolism
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Protein Kinase C beta
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Protein Tyrosine Phosphatases / metabolism*
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Receptor-Like Protein Tyrosine Phosphatases, Class 2
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Receptor-Like Protein Tyrosine Phosphatases, Class 8
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Receptors for Activated C Kinase
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Receptors, Cell Surface
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Tetraspanin 24
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Trans-Activators / metabolism
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beta Catenin
Substances
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Antigens, CD
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CD151 protein, human
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CTNNB1 protein, mouse
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Cadherins
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Cd151 protein, mouse
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Cell Adhesion Molecules
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Cytoskeletal Proteins
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Integrin alpha3beta1
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Laminin
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Macromolecular Substances
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Neoplasm Proteins
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RACK1 protein, human
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Receptors for Activated C Kinase
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Receptors, Cell Surface
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Recombinant Fusion Proteins
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Tetraspanin 24
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Trans-Activators
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beta Catenin
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laminin 1
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Protein Kinase C
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Protein Kinase C beta
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PTPRN protein, human
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Protein Tyrosine Phosphatases
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Ptprn protein, mouse
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Receptor-Like Protein Tyrosine Phosphatases, Class 2
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Receptor-Like Protein Tyrosine Phosphatases, Class 8
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GTP-Binding Proteins