The murine alpha-lactalbumin-encoding gene (m alpha La) was isolated and completely sequenced. The 2.3-kb transcription unit shared a similar organization with that of its counterparts from other species. Sequence comparison for the proximal 5'-flanking region indicated the presence of a consensus motif that occurs in all milk-protein-encoding genes, except the kappa-casein-encoding gene. This may correspond to the binding site for the recently identified mammary-gland-specific factor. The m alpha La gene occurs in a single copy per haploid genome and is specifically expressed in the mammary gland where it is induced during late pregnancy.