Identification and characterization of Mlr1,2: two mouse homologues of Mblk-1, a transcription factor from the honeybee brain(1)

FEBS Lett. 2003 Jan 30;535(1-3):61-5. doi: 10.1016/s0014-5793(02)03858-9.

Abstract

We previously identified the Mblk-1 gene in the honeybee brain, which encodes a transcription factor containing two DNA binding motifs, termed RHF1 and 2 (Takeuchi et al. (2001) Insect Mol. Biol. 121, 134-140). Here, we identified two mouse Mblk1 homologues, Mlr1 and Mlr2. Both encode proteins containing a single DNA-binding motif highly conserved with RHF2 and activate transcription mediated by a DNA element recognized by honeybee Mblk-1. Mlr1 was expressed predominantly in the spermatocytes of the testis, while Mlr2 was expressed in various tissues other than testis. Mlr1 transcripts were lost in the testis of W/W(v) mutant mice, suggesting a role in spermatogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Bees
  • Brain Chemistry*
  • Conserved Sequence
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Insect Proteins / genetics*
  • Male
  • Mice
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Organ Specificity
  • RNA, Messenger / biosynthesis
  • Response Elements / physiology
  • Sequence Homology, Amino Acid
  • Spermatocytes / metabolism
  • Testis / cytology
  • Testis / metabolism
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*

Substances

  • DNA, Complementary
  • Insect Proteins
  • Mlr1 protein, mouse
  • Mlr2 protein, mouse
  • RNA, Messenger
  • Transcription Factors

Associated data

  • GENBANK/AB076078
  • GENBANK/AB076079