Abstract
We previously identified the Mblk-1 gene in the honeybee brain, which encodes a transcription factor containing two DNA binding motifs, termed RHF1 and 2 (Takeuchi et al. (2001) Insect Mol. Biol. 121, 134-140). Here, we identified two mouse Mblk1 homologues, Mlr1 and Mlr2. Both encode proteins containing a single DNA-binding motif highly conserved with RHF2 and activate transcription mediated by a DNA element recognized by honeybee Mblk-1. Mlr1 was expressed predominantly in the spermatocytes of the testis, while Mlr2 was expressed in various tissues other than testis. Mlr1 transcripts were lost in the testis of W/W(v) mutant mice, suggesting a role in spermatogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Bees
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Brain Chemistry*
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Conserved Sequence
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Insect Proteins / genetics*
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Male
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Mice
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Mice, Mutant Strains
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Molecular Sequence Data
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Organ Specificity
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RNA, Messenger / biosynthesis
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Response Elements / physiology
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Sequence Homology, Amino Acid
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Spermatocytes / metabolism
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Testis / cytology
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Testis / metabolism
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Transcription Factors / biosynthesis
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Transcription Factors / genetics*
Substances
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DNA, Complementary
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Insect Proteins
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Mlr1 protein, mouse
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Mlr2 protein, mouse
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RNA, Messenger
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Transcription Factors
Associated data
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GENBANK/AB076078
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GENBANK/AB076079