Abstract
We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Chick Embryo
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Cloning, Molecular
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DNA, Complementary / biosynthesis
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DNA, Complementary / chemistry
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DNA, Complementary / isolation & purification
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Drosophila Proteins*
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In Situ Hybridization
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Limb Buds
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Mandible / embryology
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Mandible / metabolism
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Molecular Sequence Data
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis*
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RNA-Binding Proteins / biosynthesis
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / genetics*
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Ribonucleoproteins / chemistry
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Ribonucleoproteins / genetics*
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Sequence Alignment
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Telencephalon / embryology
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Telencephalon / metabolism
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Wings, Animal / embryology
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Wings, Animal / metabolism
Substances
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DNA, Complementary
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Drosophila Proteins
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RNA, Messenger
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RNA-Binding Proteins
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Ribonucleoproteins
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tra2 protein, Drosophila