Abstract
A novel cytokine termed IL-17D was cloned using nested RACE PCR. It is a secreted cytokine with homology to the IL-17 family of proteins. IL-17D is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas. Treatment of endothelial cells with purified rIL-17D protein stimulated the production of IL-6, IL-8, and GM-CSF. The increased expression of IL-8 was found to be NF-kappa B-dependent. rIL-17D also demonstrated an inhibitory effect on hemopoiesis of myeloid progenitor cells in colony formation assays.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Motifs
-
Amino Acid Sequence
-
Bone Marrow Cells / cytology
-
Bone Marrow Cells / immunology
-
Cell Line
-
Cells, Cultured
-
Cloning, Molecular
-
Colony-Forming Units Assay
-
Conserved Sequence
-
Cysteine / chemistry
-
Cytokines / biosynthesis*
-
Growth Inhibitors / isolation & purification*
-
Growth Inhibitors / physiology*
-
Hematopoiesis / immunology*
-
Humans
-
Interleukin-17 / biosynthesis
-
Interleukin-17 / genetics
-
Interleukin-17 / isolation & purification*
-
Interleukin-17 / physiology*
-
Molecular Sequence Data
-
Multigene Family / immunology*
-
Myeloid Progenitor Cells / cytology
-
Myeloid Progenitor Cells / immunology
Substances
-
Cytokines
-
Growth Inhibitors
-
IL17D protein, human
-
Interleukin-17
-
Cysteine