Abstract
B144/LST1 is a gene encoded in the human major histocompatibility complex that produces multiple forms of alternatively spliced mRNA and encodes peptides fewer than 100 amino acids in length. B144/LST1 is strongly expressed in dendritic cells. Transfection of B144/LST1 into a variety of cells induces morphologic changes including the production of long, thin filopodia differing from those seen on transfection of a dominant active CDC42 gene. The structures are dynamically rearranging and sometimes connect one cell with another. The full effect of B144/LST1 protein on cell morphology requires the retention of at least one of the four cysteines of the peptide plus the presence of a hydrophobic segment in the protein, but requires only one of the two coding regions present in the terminal 3' exons.
Copyright 2001 Academic Press.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Blood Proteins / biosynthesis
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Blood Proteins / genetics*
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COS Cells
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Cell Differentiation
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Cell Division
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Conserved Sequence
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Cytoplasmic Granules
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Dendritic Cells / cytology
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Evolution, Molecular
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Humans
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Immune System / cytology
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Intracellular Signaling Peptides and Proteins
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Major Histocompatibility Complex / genetics*
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Membrane Proteins
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Mice
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Morphogenesis
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Multigene Family*
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Nerve Tissue Proteins / metabolism
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Pseudopodia / genetics*
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Recombinant Proteins / biosynthesis
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Tumor Necrosis Factor-alpha / genetics*
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Wiskott-Aldrich Syndrome Protein, Neuronal
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cdc42 GTP-Binding Protein / metabolism
Substances
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Blood Proteins
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Intracellular Signaling Peptides and Proteins
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LST1 protein, human
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Lst1 protein, mouse
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Membrane Proteins
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Nerve Tissue Proteins
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Recombinant Proteins
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Tumor Necrosis Factor-alpha
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WASL protein, human
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Wasl protein, mouse
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Wiskott-Aldrich Syndrome Protein, Neuronal
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cdc42 GTP-Binding Protein