The origin and expansion of hematopoietic progenitor and stem cells during fetal development and their differentiation into mature effector cells are thought to be driven by the activation of developmental stage- and cell-lineage-specific genes. To gain further insight into the molecular mechanisms regulating the expansion and differentiation of fetal hematopoietic progenitor and stem cells, we performed differential display RT-PCR analysis on fractionated murine E12 fetal liver cells. We identified a novel transcript predicted to encode a protein of 305 amino acids with a calculated molecular mass of 35 kDa, containing a charged domain and three putative C(3)H-type zinc fingers. The fetal liver zinc-finger protein 1 (Fliz1) transcript is approximately 1.8 kb and is variably expressed both during embryogenesis and in adult tissues. Fliz1 expression was detected in discrete cell foci in the fetal liver and in LIN(-)/ckit(+) cells. Nuclear localization studies revealed that Fliz1 is targeted to the nucleus. Thus, Fliz1 is a newly identified nuclear protein expressed in hematopoietic progenitor cells of the developing fetal liver.
Copyright 2001 Academic Press.