Molecular cloning of a novel ubiquitin-like protein, UBIN, that binds to ER targeting signal sequences

Biochem Biophys Res Commun. 2001 Jan 19;280(2):535-40. doi: 10.1006/bbrc.2000.4149.

Abstract

To identify proteins that interact with HSP47, an endoplasmic reticulum (ER)-resident molecular chaperone, a yeast two-hybrid screening was performed using mouse full-length HSP47 including an N-terminal signal sequence as a bait. Analysis of several positive clones led to the identification and cloning of a novel gene, ubin, encoding a ubiquitin-like protein. Unlike other ubiquitin-like proteins, UBIN was shown to interact with signal sequences of various secretory and ER-luminal proteins, including HSP47, but not interact with signal sequences of mitochondrial targeting in two-hybrid system. The possible function of UBIN will be discussed with regards to novel characteristics of binding to signal sequences for ER targeting.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Autophagy-Related Proteins
  • Binding Sites
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Endoplasmic Reticulum / metabolism*
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Profiling
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Protein Binding
  • Protein Sorting Signals / genetics
  • Protein Sorting Signals / physiology*
  • Protein Transport
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • Sequence Deletion / genetics
  • Tumor Cells, Cultured
  • Two-Hybrid System Techniques
  • Ubiquitins / chemistry
  • Ubiquitins / genetics*
  • Ubiquitins / metabolism*

Substances

  • Autophagy-Related Proteins
  • Carrier Proteins
  • Nuclear Proteins
  • Protein Sorting Signals
  • RNA, Messenger
  • UBIN protein, mouse
  • UBQLN4 protein, human
  • Ubiquitins

Associated data

  • GENBANK/AB040050