Abstract
Previous work demonstrated that a single pre-mRNA could generate multiple forms of mammalian poly(A) polymerase mRNAs by alternative splicing or alternative polyadenylation. A cDNA encoding a testis-specific poly(A) polymerase was isolated in this study. The transcription level of Papt in testis of a 2 weeks old mouse was much lower than that of the general poly(A) polymerase gene, Pap. However, the transcription ratio of Papt to Pap was reversed in testis of a 4 weeks old mouse. Transient expression analysis showed that GFP-Papt fusion protein is present both in the nucleus and cytoplasm of HeLa cells. These results suggest that Papt is involved in polyadenylation of transcripts expressed during spermatogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Cell Nucleus / enzymology
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Cytoplasm / enzymology
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Gene Library
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Green Fluorescent Proteins
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HeLa Cells
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Humans
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Introns
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Luminescent Proteins / analysis
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Luminescent Proteins / genetics
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Male
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Polynucleotide Adenylyltransferase / analysis
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Polynucleotide Adenylyltransferase / genetics*
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / analysis
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Restriction Mapping
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Homology, Amino Acid
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Testis / enzymology*
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Transcription, Genetic
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Transfection
Substances
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Luminescent Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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Polynucleotide Adenylyltransferase