Mycobacterium tuberculosis-activated dendritic cells induce protective immunity in mice

Immunology. 2000 Mar;99(3):473-80. doi: 10.1046/j.1365-2567.2000.00963.x.

Abstract

Activated dendritic cells are critically important in the priming of T-cell responses. In this report we show that the infection of a conditionally immortalized dendritic cell line (tsDC) with Mycobacterium tuberculosis resulted in the up-regulation of B7-1 and B7-2 co-stimulatory molecules and the induction of several inflammatory cytokines, including tumour necrosis factor-alpha and interleukin-6, -1beta and -12. In addition, we show that these activated dendritic cells were capable of eliciting antigen-specific T-cell responses and potent anti-mycobacterial protective immunity in a murine model of experimental tuberculosis infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antigens, CD / analysis*
  • B7-1 Antigen / analysis
  • B7-2 Antigen
  • Cytokines / immunology*
  • Dendritic Cells / immunology*
  • Dendritic Cells / microbiology
  • Dendritic Cells / ultrastructure
  • Female
  • Interleukin-1 / genetics
  • Interleukin-12 / genetics
  • Interleukin-6 / genetics
  • Macrophages, Peritoneal / immunology
  • Membrane Glycoproteins / analysis
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Microscopy, Electron
  • Mycobacterium tuberculosis*
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes / immunology
  • Tuberculosis / immunology*
  • Tuberculosis / pathology
  • Tuberculosis / prevention & control
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / genetics

Substances

  • Antigens, CD
  • B7-1 Antigen
  • B7-2 Antigen
  • Cd86 protein, mouse
  • Cytokines
  • Interleukin-1
  • Interleukin-6
  • Membrane Glycoproteins
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Interleukin-12