Abstract
Activated dendritic cells are critically important in the priming of T-cell responses. In this report we show that the infection of a conditionally immortalized dendritic cell line (tsDC) with Mycobacterium tuberculosis resulted in the up-regulation of B7-1 and B7-2 co-stimulatory molecules and the induction of several inflammatory cytokines, including tumour necrosis factor-alpha and interleukin-6, -1beta and -12. In addition, we show that these activated dendritic cells were capable of eliciting antigen-specific T-cell responses and potent anti-mycobacterial protective immunity in a murine model of experimental tuberculosis infection.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adoptive Transfer
-
Animals
-
Antigens, CD / analysis*
-
B7-1 Antigen / analysis
-
B7-2 Antigen
-
Cytokines / immunology*
-
Dendritic Cells / immunology*
-
Dendritic Cells / microbiology
-
Dendritic Cells / ultrastructure
-
Female
-
Interleukin-1 / genetics
-
Interleukin-12 / genetics
-
Interleukin-6 / genetics
-
Macrophages, Peritoneal / immunology
-
Membrane Glycoproteins / analysis
-
Mice
-
Mice, Inbred C57BL
-
Mice, Inbred CBA
-
Microscopy, Electron
-
Mycobacterium tuberculosis*
-
RNA, Messenger / analysis
-
Reverse Transcriptase Polymerase Chain Reaction
-
T-Lymphocytes / immunology
-
Tuberculosis / immunology*
-
Tuberculosis / pathology
-
Tuberculosis / prevention & control
-
Tumor Cells, Cultured
-
Tumor Necrosis Factor-alpha / genetics
Substances
-
Antigens, CD
-
B7-1 Antigen
-
B7-2 Antigen
-
Cd86 protein, mouse
-
Cytokines
-
Interleukin-1
-
Interleukin-6
-
Membrane Glycoproteins
-
RNA, Messenger
-
Tumor Necrosis Factor-alpha
-
Interleukin-12