T-STAR/ETOILE: a novel relative of SAM68 that interacts with an RNA-binding protein implicated in spermatogenesis

Hum Mol Genet. 1999 Jun;8(6):959-69. doi: 10.1093/hmg/8.6.959.

Abstract

RBM is an RNA-binding protein encoded on the Y chromosome in mammals and is expressed only in the nuclei of male germ cells. Genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown. Of a number of potential partners for RBM identified by a yeast two-hybrid screen with testis cDNA, the most frequent isolates encoded a novel RNA-binding protein, termed T-STAR, that is closely related to SAM68, an Src-associated protein of unknown function. The mouse homologue was also cloned and designated étoile. It mapped to chromosome 15, while T-STAR mapped to the syntenic region on human chromosome 8. T-STAR/étoile is expressed primarily in the testis; in rat germ cells, the expression of both T-STAR/étoile and SAM68 is regulated during meiosis. Transfection of T-STAR/étoile fused with green fluorescent protein into HeLa cells caused an accumulation of protein in a novel compartment of the nucleus, adjacent to the nucleolus but distinct from the peri-nucleolar compartment. RBM and other hnRNP G family members are candidate downstream targets for regulation by T-STAR/ETOILE and SAM68.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • COS Cells
  • Cell Nucleus / chemistry
  • Chromosome Mapping
  • Chromosomes, Human, Pair 8 / genetics
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA-Binding Proteins
  • Gene Expression
  • HeLa Cells
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Muridae
  • Plasmids
  • Protein Binding
  • RNA / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • Rats
  • Saccharomyces cerevisiae / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Spermatogenesis
  • Tissue Distribution
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • DNA, Complementary
  • DNA-Binding Proteins
  • KHDRBS1 protein, human
  • KHDRBS3 protein, human
  • Khdrbs1 protein, mouse
  • Khdrbs3 protein, mouse
  • RNA, Messenger
  • RNA-Binding Proteins
  • Tumor Suppressor Protein p53
  • RNA

Associated data

  • GENBANK/AF069681
  • GENBANK/AF079763