Abstract
RBM is an RNA-binding protein encoded on the Y chromosome in mammals and is expressed only in the nuclei of male germ cells. Genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown. Of a number of potential partners for RBM identified by a yeast two-hybrid screen with testis cDNA, the most frequent isolates encoded a novel RNA-binding protein, termed T-STAR, that is closely related to SAM68, an Src-associated protein of unknown function. The mouse homologue was also cloned and designated étoile. It mapped to chromosome 15, while T-STAR mapped to the syntenic region on human chromosome 8. T-STAR/étoile is expressed primarily in the testis; in rat germ cells, the expression of both T-STAR/étoile and SAM68 is regulated during meiosis. Transfection of T-STAR/étoile fused with green fluorescent protein into HeLa cells caused an accumulation of protein in a novel compartment of the nucleus, adjacent to the nucleolus but distinct from the peri-nucleolar compartment. RBM and other hnRNP G family members are candidate downstream targets for regulation by T-STAR/ETOILE and SAM68.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Adaptor Proteins, Signal Transducing
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Amino Acid Sequence
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Animals
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Blotting, Northern
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COS Cells
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Cell Nucleus / chemistry
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Chromosome Mapping
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Chromosomes, Human, Pair 8 / genetics
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA-Binding Proteins
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Gene Expression
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HeLa Cells
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Humans
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Male
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Muridae
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Plasmids
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Protein Binding
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RNA / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA-Binding Proteins / genetics*
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RNA-Binding Proteins / metabolism
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Rats
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Saccharomyces cerevisiae / genetics
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Sequence Alignment
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Sequence Homology, Amino Acid
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Spermatogenesis
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Tissue Distribution
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism*
Substances
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Adaptor Proteins, Signal Transducing
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DNA, Complementary
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DNA-Binding Proteins
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KHDRBS1 protein, human
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KHDRBS3 protein, human
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Khdrbs1 protein, mouse
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Khdrbs3 protein, mouse
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RNA, Messenger
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RNA-Binding Proteins
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Tumor Suppressor Protein p53
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RNA
Associated data
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GENBANK/AF069681
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GENBANK/AF079763