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Series GSE3640 Query DataSets for GSE3640
Status Public on Jan 01, 2006
Title Comparative Transcriptome Profiling of Maize Lines
Organism Zea mays
Experiment type Expression profiling by array
Summary Four tissues (leaves, 1 mm anthers, 1.5 mm anthers, pollen) from one hybrid and one inbred maize line were hybridized with the W23 inbred on Agilent oligonucleotide microarrays with 21K elements. Tissue-specific gene expression patterns were documented with leaves having the most tissue-specific transcripts. Haploid pollen expresses about half as many genes as the other samples. High overlap of gene expression was found between leaves and anthers. Anther and pollen transcript expression showed high conservation among the three lines while leaves had more divergence. Antisense transcripts represented about 6 to 14 percent of total transcriptome by tissue type but were similar across lines. Gene Ontology (GO) annotations were assigned and tabulated. Enrichment in GO terms related to cell cycle functions was found for the identified antisense transcripts. Microarray results were validated via quantitative real time PCR and by hybridization to a second oligonucleotide microarray platform.
Keywords: genetic background comparison
 
Overall design We analyzed 4 tissues from 3 maize lines, each with 3 biological replicates, with W23 as a reference, and hybridized to 24 arrays.
 
Contributor(s) Ma J, Morrow DJ, Fernandes J, Walbot V
Citation(s) 16542496
Submission date Nov 20, 2005
Last update date Mar 16, 2012
Contact name Jiong Ma
E-mail(s) jiongm@stanford.edu
Organization name Stanford University
Department Biological Sciences
Lab Walbot
Street address 385 Serra Mall
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL3099 Agilent Maize 21K v1.0
Samples (24)
GSM84387 Maize 1 mm anther A619 vs W23 replicate 1
GSM84388 Maize 1 mm anther A619 vs W23 replicate 2
GSM84389 Maize 1 mm anther A619 vs W23 replicate 3
Relations
BioProject PRJNA93761

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