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Inhibition of human Sphk1 (9 to 364 residues)
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryRelated BioAssays by Target
Inhibition of SphK1 (unknown origin) expressed in Escherichia coli BL21 (DE3) using ATP as substrate preincubated for 1 hrs followed by substrate addition and measured after 30 mins by malachite green-based microtitre-plate assay
Inhibition of SphK1 (unknown origin) expressed in Escherichia coli BL21 (DE3) using ATP as substrate preincubated for 60 mins followed by substrate addition and measured after 30 mins by malachite green-based microtitre-plate assay
Assay data:1 Active, 1 Tested
Inhibition of recombinant GST-tagged SphK1 (unknown origin) (1 to 384 residues) expressed in baculovirus using sphingosine as substrate incubated for 1 hr in presence of ATP by electrophoretic mobility shift assay
Inhibition of SphK1 (unknown origin) using ATP as substrate preincubated for 40 mins followed by substrate addition and measured after 5 mins by spectrophotometric analysis
Assay data:2 Active, 1 Activity ≤ 1 µM, 3 Tested
Inhibition of SphK1 (unknown origin)
Inhibition of recombinant SphK1 (unknown origin) using NBD-sphingosine as substrate in presence of ATP by fluorescence plate reader analysis
Assay data:1 Active, 2 Tested
Inhibition of recombinant mouse SphK1 expressed in baculovirus infected Sf9 cells using D-erythro-sphingosine and [gamma-32P]ATP as substrate incubated for 30 mins by liquid scintillation counting analysis
Assay data:2 Tested
Inhibition of recombinant human SphK1 expressed in Sf9 cells assessed as decrease in [33P]SIP production using d-erythro-sphingosine and [gamma-32P]ATP as substrate by scintillation counting method
Assay data:1 Tested
Inhibition of SphK1 (unknown origin) assessed as inhibition constant
Assay data:4 Active, 2 Activity ≤ 1 µM, 5 Tested
Inhibition of recombinant human C-terminus His-tagged SphK1 expressed in baculovirus infected Sf21 cells using FITC-sphingosine as substrate preincubated for 90 mins followed by substrate addition in presence of ATP by caliper fluorescent assay
Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells using d-erythro-sphingosine and [gamma-32P]ATP as substrate by liquid scintillation counting method
Inhibition of SK1 (unknown origin)
Inhibition of recombinant mouse SphK1 transfected in HEK293T cells using D-erythro-sphingosine measured after 20 mins in presence of gamma-[p32]-ATP by autoradiography and liquid scintillation counting method
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of recombinant SPHK1 (unknown origin) expressed in baculovirus infected Sf9 insect cells using D-erythro-sphingosine as substrate incubated for 20 mins in presence of gamma-[32P]ATP by liquid scintillation counting method
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human SPHK1 by biochemical assay
Inhibition of SPHK1 (unknown origin)
Inhibition of C-terminal His6-tagged human SPHK1 expressed in baculovirus infected Sf21 insect cells using FITC-sphingosine as substrate preincubated for 90 mins followed by substrate addition in presence of ATP by caliper mobility shift assay
Inhibition of SPHK1 (unknown origin) using sphingosine-fluorescein as substrate incubated for 1 hr in presence of ATP by mobility shift assay
Assay data:12 Active, 12 Activity ≤ 1 µM, 12 Tested
Inhibition of SPHK1 (unknown origin) at 10 uM using sphingosine-fluorescein as substrate incubated for 1 hr in presence of ATP by mobility shift assay relative to control
Assay data:26 Tested
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