Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Summary
Inhibition of IMPDH2 (unknown origin)
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Enzyme Assay from Article 10.3109/14756366.2013.793184: "Design, synthesis and biological evaluation of novel inosine 5'-monophosphate dehydrogenase (IMPDH) inhibitors."
Assay data:8 Active, 4 Activity ≤ 1 µM, 48 Tested
Enzyme Inhibition Assay from Article 10.1016/j.chembiol.2007.12.010: "Targeting a prokaryotic protein in a eukaryotic pathogen: identification of lead compounds against cryptosporidiosis."
Assay data:3 Active, 4 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
IMPDH2 Enzyme Inhibition and Human T-Lymphoblast (CEM) Proliferation Inhibition Assays from Article 10.1021/jm070299x: "Acridone-based inhibitors of inosine 5'-monophosphate dehydrogenase: discovery and SAR leading to the identification of N-(2-(6-(4-ethylpiperazin-1-yl)pyridin-3-yl)propan-2-yl)-2- fluoro-9-oxo-9,10-dihydroacridine-3-carboxamide (BMS-566419)."
Assay data:26 Active, 25 Activity ≤ 1 µM, 26 Tested
IMPDH Type 2 Enzyme Assay from Article 10.1021/jm070568j: "Probing Binding Requirements of Type I and Type II Isoforms of Inosine Monophosphate Dehydrogenase with Adenine-Modified Nicotinamide Adenine Dinucleotide Analogues."
Assay data:14 Active, 14 Activity ≤ 1 µM, 14 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
IMPDH Type 1 Enzyme Assay from Article 10.1021/jm070568j: "Probing Binding Requirements of Type I and Type II Isoforms of Inosine Monophosphate Dehydrogenase with Adenine-Modified Nicotinamide Adenine Dinucleotide Analogues."
Assay data:14 Active, 1 Activity ≤ 1 nM, 14 Activity ≤ 1 µM, 14 Tested
Inhibition of IMPDH2 (unknown origin) assessed by conversion of NAD+ to NADH preincubated for 30 mins followed by addition of inosine-5'- monophosphate substrate and NAD at 10 uM measured by plate reader method
Assay data:1 Active, 5 Tested
Inhibition of human IMPDH2 expressed in Escherichia coli assessed as apparent inhibition constant using NAD as substrate by fluorescence assay
Assay data:13 Active, 12 Activity ≤ 1 µM, 17 Tested
Binding affinity to IMPDH2 in human HeLa cell lysates assessed as cysteine-compound adduct formation at 50 uM preincubated for 22 mins followed by IAA-probe addition and measured after 20 mins by mass spectrometric method
Assay data:1 Active, 1 Tested
Inhibition of human IMPDH2 relative to control
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to IMPDH2 (unknown origin)
Assay data:1 Tested
Binding affinity to IMPDH2 in human NCI-H358 cells at 1 uM by mass spectrometry based pull down assay
Binding affinity to IMPDH2 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay
Binding affinity to IMPDH1 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay
Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry.
Assay data:1 Active, 1 Activity ≤ 1 µM, 209 Tested
Inhibition of recombinant human His-tagged IMPDH2 expressed in Escherichia coli BL21 (DE3) using IMP as substrate in presence of NAD
Assay data:14 Active, 1 Activity ≤ 1 µM, 16 Tested
Inhibition of recombinant human His-tagged IMPDH1 expressed in Escherichia coli BL21 (DE3) using IMP as substrate in presence of NAD
Assay data:8 Active, 1 Activity ≤ 1 µM, 16 Tested
Inhibition of human IMPDH2 using IMP as substrate in presence of NAD+ after 30 mins
Assay data:9 Active, 7 Activity ≤ 1 µM, 38 Tested
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on