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Caspase Glo MOLP-8 assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 2 Tested
SummaryRelated BioAssays by Target
HTRF (MCL1)
Assay data:1 Active, 1 Activity ≤ 1 nM, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of N-terminal GST-tagged human recombinant Mcl-1 expressed in Escherichia coli /human Bim measured for 60 mins by by TR-FRET based LanthaScreen Eu-Kinase binding assay
Assay data:26 Active, 17 Activity ≤ 1 µM, 29 Tested
Binding affinity to N-terminal his6-tagged human recombinant Mcl-1 expressed in Escherichia coli assessed as dissociation constant by SPR analysis
Assay data:16 Active, 12 Activity ≤ 1 µM, 17 Tested
Binding affinity to N-terminal his6-tagged human recombinant Mcl-1 expressed in Escherichia coli assessed as binding constant (Kon) by SPR analysis
Assay data:9 Tested
Binding affinity to N-terminal his6-tagged human recombinant Mcl-1 expressed in Escherichia coli assessed as binding constant (Koff) by SPR analysis
Binding affinity to Mcl-1 (unknown origin)
Assay data:3 Active, 3 Activity ≤ 1 nM, 3 Activity ≤ 1 µM, 3 Tested
Inhibition of TAMRA-labeled BIM-BH3 peptide binding to 6His-tagged human recombinant Mcl-1 (171 to 327 residues) incubated for 120 mins by fluorescence polarization assay
Assay data:5 Active, 1 Activity ≤ 1 nM, 3 Activity ≤ 1 µM, 5 Tested
Inhibition of FITC-labeled BID-BH3 peptide binding to Mcl-1 (unknown origin) by fluorescence polarization assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of fluorescent-labeled BID-BH3 peptide binding to Mcl-1 (unknown origin) incubated for 20 mins by fluorescence polarization assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of fluorescent-labeled BID-BH3 peptide binding to Mcl-1 (unknown origin) by fluorescence polarization assay
Displacement of fluorescein tagged BID BH3 peptide from GST-tagged recombinant Mcl-1 (unknown origin) by fluorescence polarization-based competitive binding assay
Inhibition of Mcl-1 (unknown origin)
Displacement of fluorescein tagged Bak BH3 peptide from MCL-1 (172 to 327 residues) (unknown origin) by fluorescence polarization competition assay
Assay data:1 Active, 1 Tested
Inhibition of FITC-labeled BH3 peptide binding to MCL1 (unknown origin)
Assay data:1 Tested
Antagonist activity at human his 6- trx tagged MCL1-Bim BH3 peptide by fluorescence polarization competition assay
Displacement of fluorescent-labeled BH3 peptide from recombinant MCL1 (unknown origin) by fluorescence polarization-based competitive binding assay
Assay data:3 Active, 3 Tested
Binding affinity to fluorescent-labeled BH3 peptide from GST tagged MCL1 (172 to 320 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) incubated for 15 mins by fluorescence polarization-based competitive binding assay
Binding affinity to Mcl-1 (unknown origin) assessed as inhibition constant by TR-FRET assay
Assay data:1 Activity ≤ 1 µM, 1 Tested
Binding affinity to Mcl-1 (unknown origin) assessed as inhibition constant
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