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Antagonist activity at human NPFF2R expressed in HEK293 cells upto 10 uM
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Agonist activity at human NPFF2R expressed in HEK293 cells upto 10 uM
Displacement of [125I]-1DMeNPFF from mouse NPFF2R receptor by TopCount scintillation counting method
Assay data:5 Active, 2 Activity ≤ 1 µM, 6 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Displacement of [125I]-1DMeNPFF from recombinant human NPFF2 receptor expressed in CHO cells by TopCount scintillation counting method
Assay data:24 Active, 2 Activity ≤ 1 nM, 14 Activity ≤ 1 µM, 24 Tested
Agonist activity at NPFF2R (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-induced cAMP accumulation by measuring maximal efficacy preincubated for 30 mins in presence of 3-isobutyl-1-methylxanthine followed by forskolin and compound addition by competitive PKA binding assay relative to control
Assay data:9 Tested
Agonist activity at NPFF2R (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins in presence of 3-isobutyl-1-methylxanthine followed by forskolin and compound addition by competitive PKA binding assay
Assay data:9 Active, 9 Activity ≤ 1 µM, 9 Tested
PRESTO-Tango GPCRome screening (NPFF2)
SummaryRelated BioAssays by Target
Antagonist activity at human NPFFR2 expressed in CHO-K1 cells assessed as apparent dissociation constant Ke by Eu-cAMP tracer based TR-FRET assay
Assay data:2 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to human NPFFR2
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 1 Tested
Displacement of [3H]-FFRF-NH2 from human NPFFR2 expressed in CHO cell membrane by Topcount scintillation counting method
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Antagonist activity at human NPFFR2 expressed in CHO-RD-HGA16 cells assessed as reduction in NPFF-induced calcium influx by measuring apparent dissociation constant Ke preincubated for 15 mins followed by NPFF addition by calcium-5 dye based fluorescence method
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Displacement of [125I]NPFF from human NPFFR2 expressed in CHO cell membranes incubated for 120 mins by scintillation/luminescence counting method
Displacement of [3H]-FFRF-NH2 from human FLAG-tagged NPFFR2 expressed in CHO cell membrane incubated for 30 mins by Topcount scintillation counting method
Agonist activity at human NPFFR2 expressed in CHO cells assessed as inhibition of forskolin-induced intracellular cAMP production incubated for 5 hrs by firefly/renilla luciferase reporter assay
Displacement of TAMRA-NPFF from human NPFFR2 expressed in CHO-K1 cells preincubated for 15 mins followed by GTPgammaS addition and measured after 15 to 120 mins by competitive binding assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Displacement of 125I-NPFF from human NPFFR2 incubated for 2 hrs by TopCount scintillation counting method
Agonist activity at NPFFR2 (unknown origin) expressed in HEK293T cells harboring EP2 incubated for 15 mins by cAMP assay
Assay data:3 Active, 3 Tested
Agonist activity at NPFFR2 (unknown origin) expressed in HEK293T cells harboring EP2 incubated for 15 mins by cAMP assay relative to control
Assay data:4 Tested
Agonist activity at NPFFR2 (unknown origin) expressed in mouse NIH3T3 cells by receptor selection and amplification technology assay
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Agonist activity at NPFFR2 (unknown origin) expressed in mouse NIH3T3 cells by receptor selection and amplification technology assay relative to control
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