Related BioAssays, by Target Similarity (List) for PubChem BioAssay (S - PubChem BioAssay - NCBI

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Select item 19566861.

Inhibition of human SPHK2 assessed as residual activity at 1 uM relative to control

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:1 Tested

AID:: 1956686

Summary Related BioAssays by Target

Select item 19285322.

Inhibition of human Sphk1 (9 to 364 residues)

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested

AID:: 1928532

Summary Related BioAssays by Target

Select item 19239213.

Inhibition of SphK1 (unknown origin) expressed in Escherichia coli BL21 (DE3) using ATP as substrate preincubated for 1 hrs followed by substrate addition and measured after 30 mins by malachite green-based microtitre-plate assay

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested

AID:: 1923921

Summary Related BioAssays by Target

Select item 19239204.

Inhibition of SphK1 (unknown origin) expressed in Escherichia coli BL21 (DE3) using ATP as substrate preincubated for 60 mins followed by substrate addition and measured after 30 mins by malachite green-based microtitre-plate assay

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:1 Active, 1 Tested

AID:: 1923920

Summary Related BioAssays by Target

Select item 19239195.

Inhibition of recombinant GST-tagged SphK2 (unknown origin) (1 to 618 residues) expressed in baculovirus using sphingosine as substrate incubated for 1 hr in presence of ATP by electrophoretic mobility shift assay

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:1 Active, 1 Tested

AID:: 1923919

Summary Related BioAssays by Target

Select item 19239186.

Inhibition of recombinant GST-tagged SphK1 (unknown origin) (1 to 384 residues) expressed in baculovirus using sphingosine as substrate incubated for 1 hr in presence of ATP by electrophoretic mobility shift assay

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:1 Active, 1 Tested

AID:: 1923918

Summary Related BioAssays by Target

Select item 19239177.

Inhibition of SphK2 (unknown origin) using ATP as substrate preincubated for 40 mins followed by substrate addition and measured after 5 mins by spectrophotometric analysis

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:1 Active, 2 Tested

AID:: 1923917

Summary Related BioAssays by Target

Select item 19239168.

Inhibition of SphK1 (unknown origin) using ATP as substrate preincubated for 40 mins followed by substrate addition and measured after 5 mins by spectrophotometric analysis

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:2 Active, 1 Activity ≤ 1 µM, 3 Tested

AID:: 1923916

Summary Related BioAssays by Target

Select item 19239159.

Inhibition of SphK2 (unknown origin)

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:1 Active, 1 Tested

AID:: 1923915

Summary Related BioAssays by Target

Select item 192391410.

Inhibition of SphK1 (unknown origin)

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:1 Active, 1 Tested

AID:: 1923914

Summary Related BioAssays by Target

Select item 192391311.

Inhibition of recombinant SphK2 (unknown origin) using NBD-sphingosine as substrate in presence of ATP by fluorescence plate reader analysis

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:1 Tested

AID:: 1923913

Summary Related BioAssays by Target

Select item 192391212.

Inhibition of recombinant SphK1 (unknown origin) using NBD-sphingosine as substrate in presence of ATP by fluorescence plate reader analysis

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:1 Active, 2 Tested

AID:: 1923912

Summary Related BioAssays by Target

Select item 192391113.

Inhibition of recombinant mouse SphK2 expressed in baculovirus infected Sf9 cells using D-erythro-sphingosine and [gamma-32P]ATP as substrate incubated for 30 mins by liquid scintillation counting analysis

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested

AID:: 1923911

Summary Related BioAssays by Target

Select item 192391014.

Inhibition of recombinant mouse SphK1 expressed in baculovirus infected Sf9 cells using D-erythro-sphingosine and [gamma-32P]ATP as substrate incubated for 30 mins by liquid scintillation counting analysis

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:2 Tested

AID:: 1923910

Summary Related BioAssays by Target

Select item 192390915.

Inhibition of recombinant human SphK2 expressed in Sf9 cells assessed as decrease in [33P]SIP production using d-erythro-sphingosine and [gamma-32P]ATP as substrate by scintillation counting method

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:1 Active, 1 Tested

AID:: 1923909

Summary Related BioAssays by Target

Select item 192390816.

Inhibition of recombinant human SphK1 expressed in Sf9 cells assessed as decrease in [33P]SIP production using d-erythro-sphingosine and [gamma-32P]ATP as substrate by scintillation counting method

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:1 Tested

AID:: 1923908

Summary Related BioAssays by Target

Select item 192390717.

Inhibition of SphK2 (unknown origin) assessed as inhibition constant

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:3 Active, 1 Activity ≤ 1 µM, 4 Tested

AID:: 1923907

Summary Related BioAssays by Target

Select item 192390618.

Inhibition of SphK1 (unknown origin) assessed as inhibition constant

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:4 Active, 2 Activity ≤ 1 µM, 5 Tested

AID:: 1923906

Summary Related BioAssays by Target

Select item 192390519.

Inhibition of recombinant human C-terminus His-tagged SphK2 expressed in baculovirus infected Sf21 cells using FITC-sphingosine as substrate preincubated for 90 mins followed by substrate addition in presence of ATP by caliper fluorescent assay

Source:: ChEMBL

Protein Target:: Sphingosine kinase 2

Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested

AID:: 1923905

Summary Related BioAssays by Target

Select item 192390420.

Inhibition of recombinant human C-terminus His-tagged SphK1 expressed in baculovirus infected Sf21 cells using FITC-sphingosine as substrate preincubated for 90 mins followed by substrate addition in presence of ATP by caliper fluorescent assay

Source:: ChEMBL

Protein Target:: Sphingosine kinase 1

Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested

AID:: 1923904

Summary Related BioAssays by Target

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BioAssays with protein sequences specified as targets

BioActivity

Active Compounds (182)
BioAssays with compounds reported as biologically active
Activity (IC50, etc) ≤ 1 nM (3)
BioAssays with the active concentration of tested reagents less than 1 nanomolar
Activity (IC50, etc) ≤ 1 µM (79)
BioAssays with the active concentration of tested reagents less than 1 micromolar

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Low-throughput BioAssays where the activity outcome is based on a dose-response relationship with multiple tested concentrations

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BioAssays extracted from the literature
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Related BioAssays, by Target Similarity (List) for PubChem BioAss... (404)
Related BioAssays, by Target Similarity (List) for PubChem BioAssay (Select 1794980)
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PubChem BioAssay
Rattus norvegicus protein tyrosine phosphatase, receptor type, C (Ptprc), transc...
Rattus norvegicus protein tyrosine phosphatase, receptor type, C (Ptprc), transcript variant 4, mRNA
gi|1050115293|ref|NM_138507.3|

Nucleotide
Chromosome neighbors for GEO Profiles (Select 38810433) (14)
GEO Profiles
Gene Links for GEO Profiles (Select 106774327) (1)
Gene
Afap1 actin filament associated protein 1 [Mus musculus]
Afap1 actin filament associated protein 1 [Mus musculus]
Gene ID:70292

Gene

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